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MCQs

Total Questions : 48 | Page 5 of 5 pages
Question 41. . What would happen if serum is omitted from the ELISA, but all other steps remain same?
  1.    Anti-human Ig-conjugate would not bind and be washed away
  2.    The OD values would be nearly the same as the assay control
  3.    Both (a) and (b)
  4.    Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
 Discuss Question
Answer: Option C. -> Both (a) and (b)
Question 42. . What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
  1.    The ELISA would not develop when the substrate was added
  2.    The ELISA would develop normally
  3.    All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
  4.    None of the above
 Discuss Question
Answer: Option C. -> All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
Question 43. . An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that
  1.    there was more than one antigen
  2.    the antibody was impure
  3.    the temperature was too high
  4.    there was one antibody and one antigen
 Discuss Question
Answer: Option A. -> there was more than one antigen
Question 44. . Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis
  1.    prior to reaction with antibody
  2.    prior to reaction with antigen
  3.    after reaction with antibody
  4.    after reaction with antigen
 Discuss Question
Answer: Option A. -> prior to reaction with antibody
Question 45. . Which of the following is a positively charged matrix for ion exchange chromatography?
  1.    CM cellulose
  2.    DEAE cellulose
  3.    Phosphocellulose
  4.    None of these
 Discuss Question
Answer: Option B. -> DEAE cellulose
Question 46. . Which of the following is an immunoelectrophoresis technique?
  1.    Two-dimensional immunoelectrophoresis
  2.    Counterimmunoelectrophoresis
  3.    Coimmunoelectrophoresis
  4.    Both (a) and (b)
 Discuss Question
Answer: Option D. -> Both (a) and (b)
Question 47. . Which of the following is a positively charged matrix for ion exchange chromatography?
  1.    CM cellulose
  2.    DEAE cellulose
  3.    Phosphocellulose
  4.    None of these
 Discuss Question
Answer: Option B. -> DEAE cellulose
Question 48. . Which of the following is an immunoelectrophoresis technique?
  1.    Two-dimensional immunoelectrophoresis
  2.    Counterimmunoelectrophoresis
  3.    Coimmunoelectrophoresis
  4.    Both (a) and (b)
 Discuss Question
Answer: Option D. -> Both (a) and (b)

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