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MCQs

Total Questions : 48 | Page 3 of 5 pages
Question 21. Which of the following is an immunoelectrophoresis technique?
  1.    Two-dimensional immunoelectrophoresis
  2.    Coimmunoelectrophoresis
  3.    Counterimmunoelectrophoresis
  4.    Both (a) and (b)
 Discuss Question
Answer: Option D. -> Both (a) and (b)
Question 22. Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis
  1.    prior to reaction with antibody
  2.    prior to reaction with antigen
  3.    after reaction with antibody
  4.    after reaction with antigen
 Discuss Question
Answer: Option A. -> prior to reaction with antibody
Question 23. Which of the following is a positively charged matrix for ion exchange chromatography?
  1.    Phosphocellulose
  2.    CM cellulose
  3.    DEAE cellulose
  4.    None of these
 Discuss Question
Answer: Option C. -> DEAE cellulose
Question 24.  Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively
  1.    2000 kb and 20kb DNA
  2.    1000 kb and l0kb DNA
  3.    20 kb and 2000 kb DNA
  4.    10 kb and 1000 kb DNA
 Discuss Question
Answer: Option C. -> 20 kb and 2000 kb DNA
Question 25.  The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?
  1.    Blue
  2.    White
  3.    Brown
  4.    No colour is produced
 Discuss Question
Answer: Option A. -> Blue
Question 26.  The competitive immunoassay can be used
  1.    to detect very small amounts of antigen
  2.    to detect antibody associated with allergies
  3.    both (a) and (b)
  4.    commonly to detect trace amounts of drugs.
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 27.  Which of the following is an immunodiffusion test?
  1.    Double-diffusion
  2.    Gel diffusion
  3.    Ouchterloney technique
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 28.  In Counter immunoelectrophoresis
  1.    electrophoresis will drive the antibody and antigen parallel to each other
  2.    electrophoresis will drive the antibody and antigen toward each other
  3.    the antibody will migrate towards anode
  4.    the antibody will migrate towards cathode
 Discuss Question
Answer: Option B. -> electrophoresis will drive the antibody and antigen toward each other
Question 29.  What is the ELISA test intended to measure?
  1.    Antibody to HIV only
  2.    Antigen to HIV only
  3.    Presence of free, circulating virus in the patient
  4.    Antibodies directed against HLA molecules
 Discuss Question
Answer: Option A. -> Antibody to HIV only
Question 30.  Immunoaffinity chromatography can be used in biochemical applications to
  1.    break down antibody structure
  2.    purify protein antigen
  3.    break down antigen and analyze quantitatively
  4.    none of the above
 Discuss Question
Answer: Option B. -> purify protein antigen

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