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Question
. What would happen if serum is omitted from the ELISA, but all other steps remain same?
Options:
A .  Anti-human Ig-conjugate would not bind and be washed away
B .  The OD values would be nearly the same as the assay control
C .  Both (a) and (b)
D .  Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Answer: Option C

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