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MCQs

Total Questions : 48 | Page 2 of 5 pages
Question 11. Agarose a porous matrix used in various molecular biology technique is made by repetitive units of
  1.    agar
  2.    none of these
  3.    agarobiose
  4.    maltose
 Discuss Question
Answer: Option D. -> maltose
Question 12. What is approximatively the half life of 32-Phosphorus?
  1.    42 days
  2.    30 days
  3.    14 days
  4.    28 days
 Discuss Question
Answer: Option C. -> 14 days
Question 13. What radioactive molecule is used to end-label an oligonucleotide?
  1.    Any of these
  2.    (alpha)-32P-ATP
  3.    (gamma)-32P-ATP
  4.    (alpha)-32P-dUTP
 Discuss Question
Answer: Option C. -> (gamma)-32P-ATP
Question 14. Which of the following radioactive element is generally involved in RIA?
  1.    Carbon-14
  2.    Tritium
  3.    Iodine-125
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 15. Rocket Immunodiffusion is also known as
  1.    gel diffusion
  2.    electroimmunodiffusion
  3.    double-diffusion
  4.    none of these
 Discuss Question
Answer: Option B. -> electroimmunodiffusion
Question 16. RAST test (radio allergosorbent test) is often used to detect
  1.    None of these
  2.    bacteriophages
  3.    antibodies associated with allergies (IgE)
  4.    antigen associated with allergies (IgE)
 Discuss Question
Answer: Option C. -> antibodies associated with allergies (IgE)
Question 17. What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
  1.    The ELISA would develop normally
  2.    The ELISA would not develop when the substrate was added
  3.    None of these
  4.    All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
 Discuss Question
Answer: Option D. -> All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
Question 18. An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that
  1.    the temperature was too high
  2.    there was more than one antigen
  3.    the antibody was impure
  4.    there was one antibody and one antigen
 Discuss Question
Answer: Option B. -> there was more than one antigen
Question 19. DNA- footprinting is a suitable technique for identifying which of the following?
  1.    Protein binding site within DNA
  2.    Particular t-RNA in mixture
  3.    Introns within DNA
  4.    Particular mRNA in mixture
 Discuss Question
Answer: Option A. -> Protein binding site within DNA
Question 20. What would happen if serum is omitted from the ELISA, but all other steps remain same?
  1.    Both (a) and (b)
  2.    The OD values would be nearly the same as the assay control
  3.    Anti-human Ig-conjugate would not bind and be washed away
  4.    Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
 Discuss Question
Answer: Option A. -> Both (a) and (b)

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