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Question
. What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
Options:
A .  The ELISA would not develop when the substrate was added
B .  The ELISA would develop normally
C .  All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
D .  None of the above
Answer: Option C

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