What would happen if serum is omitted from the ELISA, but all other steps remain same? Options: A . &nbspBoth (a) and (b) B . &nbspThe OD values would be nearly the same as the assay control C . &nbspAnti-human Ig-conjugate would not bind and be washed away D . &nbspAnti-human Ig-conjugate would bind non-specifically to the ELISA plate

What would happen if serum is omitted from the ELISA, but all other steps remain

Question

What would happen if serum is omitted from the ELISA, but all other steps remain same?

Options:

A . Both (a) and (b)

B . The OD values would be nearly the same as the assay control

C . Anti-human Ig-conjugate would not bind and be washed away

D . Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

Answer: Option A

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