What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? Options: A . &nbspThe ELISA would develop normally B . &nbspThe ELISA would not develop when the substrate was added C . &nbspNone of these D . &nbspAll wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

What would happen if the anti-human Ig-conjugate is not washed free of the well

Question

What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

Options:

A . The ELISA would develop normally

B . The ELISA would not develop when the substrate was added

C . None of these

D . All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

Answer: Option D

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