Questions and answers for exam Preparation

MCQs

Total Questions : 165 | Page 10 of 17 pages

Question 91. Which of the following is untrue about DNA sequencing methods?

Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)
Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer
Taq polymerase is quite heat sensitive
New strands of DNA are synthesized from the end of the primer

Discuss Question

Answer: Option C. -> Taq polymerase is quite heat sensitive
Answer: (c).Taq polymerase is quite heat sensitive

Question 92. Using ddATP, the resulting synthesis creates a set of nested DNA fragments, each one ending at one of the as in the sequence through the substitution of a fluorescent-labeled ddATP.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 93. The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 94. The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.

separate, size
separate, pH
assimilate, pH
assimilate, size

Discuss Question

Answer: Option A. -> separate, size
Answer: (a).separate, size

Question 95. When the process is fully automated, a number of priming sites may be used to obtain sequencing results that give optimal separation of bands in each region of the sequence.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 96. The results of searches in separate fields may be combined to narrow down the choices.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 97. Knowing ________ should be enough to find the required entry quickly.

publication date, protein name, journal name
accession number, protein name, or name of gene
publication date, protein name, or volume
properties, protein name, or title word

Discuss Question

Answer: Option B. -> accession number, protein name, or name of gene
Answer: (b).accession number, protein name, or name of gene

Question 98. The program returns the number of matches found and provides an opportunity to narrow this list by including more terms.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 99. Which of the following is incorrect about ENTREZ?

There is no simple way to find the correct sequence without manually checking the information provided in each sequence, but this usually takes longer time
Before leaving ENTREZ, it is often useful to check for sequence database entries that are similar to the one of interest, called “neighbors” by ENTREZ
The expanded query searches other database entries of interest, such as the same protein in another organism, a large chromosomal sequence that includes the gene, or members of the same gene family
While visiting the site, note that ENTREZ has been adapted to search through a number of other biological databases, and also through Medline, and these searches are available from the initial ENTREZ Web page

Discuss Question

Answer: Option A. -> There is no simple way to find the correct sequence without manually checking the information provided in each sequence, but this usually takes longer time
Answer: (a).There is no simple way to find the correct sequence without manually checking the information provided in each sequence, but this usually takes longer time

Question 100. Which of the following is incorrect about Retrieving a Specific Sequence?

It can be difficult to retrieve the sequence of a specific gene or protein simply because of the sheer number of sequences in the Gen-Bank database and the complex problem of indexing them
Other projects may benefit from the availability of better curated and annotated protein sequence databases, but not PIR and SwissProt
For projects that require the most currently available sequences, the NR databases should be searched
The genomic databases can also provide the sequence of a particular gene or protein. Protein sequences in the Genpro database are generated by automatic translation of DNA sequences

Discuss Question

Answer: Option B. -> Other projects may benefit from the availability of better curated and annotated protein sequence databases, but not PIR and SwissProt
Answer: (b).Other projects may benefit from the availability of better curated and annotated protein sequence databases, but not PIR and SwissProt