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MCQs

Total Questions : 238 | Page 16 of 24 pages
Question 151. In animal cell culture, particularly mammalian cell culture, transformation means
  1.    uptake of new genetic material
  2.    phenotypic modifications of cells in culture
  3.    release of genetic information
  4.    both (a) and (b)
 Discuss Question
Answer: Option B. -> phenotypic modifications of cells in culture
Question 152. Which of the following is not the explantation technique?
  1.    Adherent primary culture
  2.    Carrel flask culture
  3.    Slide culture
  4.    Roller test tube culture
 Discuss Question
Answer: Option A. -> Adherent primary culture
Question 153. Cells which have undergone transformation frequently become
  1.    anchorage independent
  2.    unstable
  3.    stable
  4.    anchorage dependent
 Discuss Question
Answer: Option A. -> anchorage independent
Question 154. The technique of organ culture may be divided on the basis of employing
  1.    semi-solid medium
  2.    both (a) and (b)
  3.    solid medium
  4.    liquid medium
 Discuss Question
Answer: Option B. -> both (a) and (b)
Question 155. Range of optimum glucose concentration present in the culture media is
  1.    75-105 mmol/litre
  2.    105-150 mmol/litre
  3.    5.5 - 55 mmol/litre
  4.    55 - 75 mmol/litre
 Discuss Question
Answer: Option C. -> 5.5 - 55 mmol/litre
Question 156. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
  1.    both (a) and (b)
  2.    none of these
  3.    oxidative pathway
  4.    anaerobic pathway
 Discuss Question
Answer: Option C. -> oxidative pathway
Question 157. An established cell line can be called where it has been subcultured at least
  1.    40 times at an interval of 3 days between subcultures
  2.    70 times at an interval of 1 day between subcultures
  3.    50 times at an interval of 3 days between subcultures
  4.    70 times at an interval of 3 days between subcultures
 Discuss Question
Answer: Option D. -> 70 times at an interval of 3 days between subcultures
Question 158. Higher dissolved oxygen concentration in the culture media are toxic and lead to
  1.    All of these
  2.    metabolism of nutrients in culture media at a rate greater than that required for consumption
  3.    DNA degradation
  4.    lipid peroxidation
 Discuss Question
Answer: Option A. -> All of these
Question 159. Which of the following is not a source of energy in active muscle cells?
  1.    Lactic acid
  2.    Creatine phosphate
  3.    Glucose
  4.    ATP
 Discuss Question
Answer: Option A. -> Lactic acid
Question 160. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
  1.    5 - 10 nmol of O2 /cell/h
  2.    5 - 10 pmol of O2/cell/h
  3.    0.05-5 pmol of O2/cell/h
  4.    0.05-5 nmol of O2/cell/h
 Discuss Question
Answer: Option C. -> 0.05-5 pmol of O2/cell/h

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