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MCQs

Total Questions : 238 | Page 17 of 24 pages
Question 161. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
  1.    established cell lines
  2.    primary cell lines
  3.    propagated cell lines
  4.    secondary cell lines
 Discuss Question
Answer: Option A. -> established cell lines
Question 162. The major problem associated with the isolation of free cells and cell aggregates from organs is that of
  1.    inhibiting the cells from their supporting matrix
  2.    None of these
  3.    disintegrating the cells from their supporting matrix
  4.    releasing the cells from their supporting matrix
 Discuss Question
Answer: Option D. -> releasing the cells from their supporting matrix
Question 163. Which of the following is the technique used for the embryo culture?
  1.    Organ cultures on agar
  2.    Whole embryo cultures
  3.    All of these
  4.    Organ cultures on plasma clots
 Discuss Question
Answer: Option C. -> All of these
Question 164. Yeast Replicating plasmids (YRp) carry
  1.    both (a) and (c)
  2.    telomere
  3.    autonomously replicating sequence
  4.    centromere
 Discuss Question
Answer: Option C. -> autonomously replicating sequence
Question 165. Which of the following viruses are used as vectors for transferring genes to animal cells?
  1.    SV 40 virus
  2.    Bovine papilloma virus (BVP)
  3.    Retroviruses
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 166. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
  1.    not necessary for viral replication
  2.    transcriptional regulators and are essential for viral replication
  3.    important for packaging of viral DNA into viral head
  4.    coding for virus coat protein
 Discuss Question
Answer: Option B. -> transcriptional regulators and are essential for viral replication
Question 167. Retroviruses have advantage for being used as vector for animal cells because
  1.    infection does not lead to cell death, infected cells produce virus over an indefinite period
  2.    they cover a wide host range
  3.    viral gene expression is driven by strong promoters
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 168. Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
  1.    either episomally or integrated at low copy number
  2.    either episomally or integrated at high copy number
  3.    episomally only
  4.    integrated only
 Discuss Question
Answer: Option B. -> either episomally or integrated at high copy number
Question 169. In case of Baculoviruses, the foreign gene
  1.    is inserted downstream of polyhedrin promoter
  2.    is inserted between the polyhedrin promoter and downstream sequences
  3.    is inserted randomly in viral genome
  4.    is inserted downstream of viral origin of replication
 Discuss Question
Answer: Option B. -> is inserted between the polyhedrin promoter and downstream sequences
Question 170. α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is
  1.    Trp 1
  2.    His 3
  3.    Lys 2
  4.    Ura 3
 Discuss Question
Answer: Option C. -> Lys 2

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