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MCQs

Total Questions : 238 | Page 18 of 24 pages
Question 171. Autonomously replicating sequence is not present in
  1.    YAC
  2.    YIp
  3.    YRp
  4.    YCp
 Discuss Question
Answer: Option B. -> YIp
Question 172. Which of the following histone is not present in SV40 virus?
  1.    H3
  2.    H1
  3.    H4
  4.    H2A
 Discuss Question
Answer: Option B. -> H1
Question 173. Yeast episomal plasmids (YEp) were constructed using
  1.    12μm yeast plasmid and E coli vector pMB 9
  2.    12μm yeast plasmid and E coli vector pBR 322
  3.    2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
  4.    2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
 Discuss Question
Answer: Option C. -> 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
Question 174. Which of the following is/are commonly used promoter of S. cerevisiae?
  1.    Alcohol dehydrogenase II (ADH 2)
  2.    Phosphoglycerate kinase (PGK)
  3.    Galactokinase (GAL 1)
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 175. Maximum amount of foreign DNA introduced into SV 40 viruses is
  1.    1 kb
  2.    2.5 kb
  3.    5 kb
  4.    10 kb
 Discuss Question
Answer: Option B. -> 2.5 kb
Question 176. Recombinant SV40 viruses introduce the foreign DNA into cells
  1.    without the RNA mediated transfection
  2.    with the DNA mediated transfection
  3.    without the DNA mediated transfection
  4.    with the RNA mediated transfection
 Discuss Question
Answer: Option C. -> without the DNA mediated transfection
Question 177. SV 40 vectors, in which early region replacements are carried out, require
  1.    pBR 322 vector
  2.    none of these
  3.    SV 40 helper virus
  4.    COS cell line
 Discuss Question
Answer: Option D. -> COS cell line
Question 178. Which one of the following is the most stable vector?
  1.    YRp
  2.    YEp
  3.    YIp
  4.    YAC
 Discuss Question
Answer: Option D. -> YAC
Question 179. Cloning genes in the yeast offer which of following advantage(s)?
  1.    Ability to glycosylate proteins during secretion
  2.    Ability to clone large piece of DNA
  3.    Over production of proteins of commercial value
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 180. Polyhedrin protein is
  1.    major component of protein matrix in which viruses are embedded
  2.    both (a) and (b)
  3.    essential for baculo virus replication
  4.    non essential for baculo virus replication
 Discuss Question
Answer: Option B. -> both (a) and (b)

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