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Total Questions : 238 | Page 23 of 24 pages
Question 221. . Cells which have undergone transformation frequently become
  1.    anchorage independent
  2.    anchorage dependent
  3.    stable
  4.    unstable
 Discuss Question
Answer: Option A. -> anchorage independent
Question 222. . Which of the following is not the explantation technique?
  1.    Slide culture
  2.    Carrel flask culture
  3.    Roller test tube culture
  4.    Adherent primary culture
 Discuss Question
Answer: Option D. -> Adherent primary culture
Question 223. . The technique of organ culture may be divided on the basis of employing
  1.    solid medium
  2.    liquid medium
  3.    both (a) and (b)
  4.    semi-solid medium
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 224. . An established cell line can be called where it has been subcultured at least
  1.    70 times at an interval of 3 days between subcultures
  2.    40 times at an interval of 3 days between subcultures
  3.    70 times at an interval of 1 day between subcultures
  4.    50 times at an interval of 3 days between subcultures
 Discuss Question
Answer: Option A. -> 70 times at an interval of 3 days between subcultures
Question 225. . Range of optimum glucose concentration present in the culture media is
  1.    5.5 - 55 mmol/litre
  2.    55 - 75 mmol/litre
  3.    75-105 mmol/litre
  4.    105-150 mmol/litre
 Discuss Question
Answer: Option A. -> 5.5 - 55 mmol/litre
Question 226. . At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
  1.    oxidative pathway
  2.    anaerobic pathway
  3.    both (a) and (b)
  4.    none of these
 Discuss Question
Answer: Option A. -> oxidative pathway
Question 227. . Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
  1.    0.05-5 nmol of O2/cell/h
  2.    5 - 10 nmol of O2 /cell/h
  3.    0.05-5 pmol of O2/cell/
  4.    5 - 10 pmol of O2/cell/h
 Discuss Question
Answer: Option C. -> 0.05-5 pmol of O2/cell/
Question 228. . Which of the following is not a source of energy in active muscle cells?
  1.    Creatine phosphate
  2.    ATP
  3.    Lactic acid
  4.    Glucose
 Discuss Question
Answer: Option C. -> Lactic acid
Question 229. . Which of the following is the technique used for the embryo culture?
  1.    Organ cultures on plasma clots
  2.    Organ cultures on agar
  3.    Whole embryo cultures
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 230. . Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
  1.    established cell lines
  2.    primary cell lines
  3.    secondary cell lines
  4.    propagated cell lines
 Discuss Question
Answer: Option A. -> established cell lines

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