Questions and answers for exam Preparation

MCQs

Total Questions : 282 | Page 27 of 29 pages

Question 261. . The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

Discuss Question

Answer: Option A. -> bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

Question 262. . X-rays cause

the formation of thymine dimers
ionization of water in the cell
heat
none of the above

Discuss Question

Answer: Option B. -> ionization of water in the cell

Question 263. . The TP53 gene of chromosome 17 codes for a protein __________ .

that plays a role in the digestive process
involved in glucose transport
involved in the regulation of the cell cycle
that is like a white blood cell protein

Discuss Question

Answer: Option C. -> involved in the regulation of the cell cycle

Question 264. . An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
different length fragments of DNA resulting from loss or gain of a restriction site
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
all of the above

Discuss Question

Answer: Option B. -> different length fragments of DNA resulting from loss or gain of a restriction site

Question 265. . Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence

Discuss Question

Answer: Option D. -> transfecting embryonic stem cells with an altered gene sequence

Question 266. . An expression vector

always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production
all of the above

Discuss Question

Answer: Option D. -> all of the above

Question 267. . Under which of the following conditions would population gene frequencies remain the same?