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Total Questions : 282 | Page 23 of 29 pages
Question 221. In order to insert a foreign gene into a plasmid, both must __________
  1.    be of the same length
  2.    be cut by the same restriction enzyme
  3.    have identical DNA sequences
  4.    originate from the same type of cell
 Discuss Question
Answer: Option B. -> be cut by the same restriction enzyme
Question 222. The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .
  1.    has occurred
  2.    seems unlikely
  3.    would be of no concern if it occurred.
  4.    can never occur
 Discuss Question
Answer: Option B. -> seems unlikely
Question 223. Which of the following techniques can be used to determine the defective gene and for developing cancer?
  1.    Southern blot
  2.    Northern blot
  3.    Western blot
  4.    Eastern blot
 Discuss Question
Answer: Option A. -> Southern blot
Question 224. Which of the following genetic diseases would be amenable to genetic engineering?
  1.    Cystic fibrosis
  2.    Muscular dystrophy
  3.    Cri du Chat
  4.    Down's syndrome
 Discuss Question
Answer: Option A. -> Cystic fibrosis
Question 225. X-rays cause
  1.    ionization of water in the cell
  2.    heat
  3.    the formation of thymine dimers
  4.    None of these
 Discuss Question
Answer: Option A. -> ionization of water in the cell
Question 226. Why is golden rice pale yellow in color?
  1.    It is rich in chlorophyll b.
  2.    It is rich in phycobilins.
  3.    It is rich in chlorophyll a.
  4.    It is rich in beta-carotene.
 Discuss Question
Answer: Option D. -> It is rich in beta-carotene.
Question 227. The order for the construction of a cDNA fragment from mRNA is to
  1.    bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
  2.    treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
  3.    digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
  4.    bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
 Discuss Question
Answer: Option D. -> bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
Question 228. The TP53 gene of chromosome 17 codes for a protein __________ .
  1.    involved in the regulation of the cell cycle
  2.    that is like a white blood cell protein
  3.    involved in glucose transport
  4.    that plays a role in the digestive process
 Discuss Question
Answer: Option A. -> involved in the regulation of the cell cycle
Question 229. Which type of restriction enzymes do not usually require ATP?
  1.    Type I
  2.    Type IV
  3.    Type II
  4.    Type III
 Discuss Question
Answer: Option C. -> Type II
Question 230. Knockout mice are created by
  1.    transfecting embryonic stem cells with an altered gene sequence
  2.    mutagenizing a mouse and selecting for mutant offspring
  3.    infecting the mouse with a retrovirus
  4.    creating a chimera by fusing cells from two different cell lines
 Discuss Question
Answer: Option A. -> transfecting embryonic stem cells with an altered gene sequence

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