Questions and answers for exam Preparation

MCQs

Total Questions : 244 | Page 22 of 25 pages

Question 211. For gene transfer to be effective, transforming DNA must be

incorporated into the bacterial chromosome
incorporated into a viral genome
free in the bacterial cytoplasm
none of the above

Discuss Question

Answer: Option A. -> incorporated into the bacterial chromosome
Answer: (a).incorporated into the bacterial chromosome

Question 212. From a single molecule of DNA, PCR can make

one additional copy
hundreds of copies
thousands of copies
millions of copies

Discuss Question

Answer: Option D. -> millions of copies
Answer: (d).millions of copies

Question 213. Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

Allosteric control of the enzyme activity
Diffusional coupling between adjacent active sites
Genetic control of the enzyme concentration
Covalent modification of the enzyme

Discuss Question

Answer: Option B. -> Diffusional coupling between adjacent active sites
Answer: (b).Diffusional coupling between adjacent active sites

Question 214. Which of the following could act as an uncoupler of electron transport and ATP synthesis?

The Fo base-piece of ATP synthase (without the Fl subunit)
Dinitrophenol
neither (a) nor (b)
Both (a) and (b)

Discuss Question

Answer: Option D. -> Both (a) and (b)
Answer: (d).Both (a) and (b)

Question 215. Genomic libraries are made from

genomic DNA of an organism
genomic RNA of an organism
genomic cDNA of an organism
genomic mRNA of an organism

Discuss Question

Answer: Option A. -> genomic DNA of an organism
Answer: (a).genomic DNA of an organism

Question 216. Phosphofructokinase, the major flux-controlling enzyme of glycolysis is allosterically inhibited and activated respectively by

ATP and PEP
AMP and Pi
ATP and ADP
Citrate and ATP

Discuss Question

Answer: Option C. -> ATP and ADP
Answer: (c).ATP and ADP

Question 217. When concentration of the reactants is higher than the equilibrium concentration then

the gibbs free energy will be positive
the gibbs free energy will be negative
more products will be formed
both (b) and (c)

Discuss Question

Answer: Option D. -> both (b) and (c)
Answer: (d).both (b) and (c)

Question 218. Which of the following is not true of glycolysis?

ADP is phosphorylated to ATP via substrate level phosphorylation
The pathway does not require oxygen
The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters
The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose

Discuss Question

Answer: Option C. -> The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters
Answer: (c).The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters

Question 219. In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because