Questions and answers for exam Preparation

MCQs

Total Questions : 35 | Page 3 of 4 pages

Question 21. ___________ catalyze the reaction by accepting a proton.

Acid
Entrapment
Enzyme
Bases

Discuss Question

Answer: Option D. -> Bases
Answer: (d).Bases

Question 22. Which of the following enzyme is assayed and measured using radioisotope method?

Hydrolases
Bacterial luciferase
Phosphofructokinase
Glutamate decarboxylase

Discuss Question

Answer: Option D. -> Glutamate decarboxylase
Answer: (d).Glutamate decarboxylase

Question 23. Which of the following is not true for acid base catalysis?

Bases catalyze the reaction by accepting a proton
Bases increases the reaction rate by increasing the nucleophilic character of the attacking group
To make reactants proximal to each other
Specific hydroxide ion catalysis of reaction in water is an example of this type of catalysis

Discuss Question

Answer: Option C. -> To make reactants proximal to each other
Answer: (c).To make reactants proximal to each other

Question 24. Which of the following is not true for strain distortion and conformational change?

Bases increases the reaction rate by increasing the nucleophilic character of the attacking group
The strain in the starting material and release of that strain provides rate acceleration in chemical reactions
This experiment was carried out by Westheimer and his colleagues
Rate of hydrolysis of phosphate esters in two compounds was explained by this theory

Discuss Question

Answer: Option A. -> Bases increases the reaction rate by increasing the nucleophilic character of the attacking group
Answer: (a).Bases increases the reaction rate by increasing the nucleophilic character of the attacking group

Question 25. Which of the following is not true for strain distortion and conformational change?

Change in conformation 3D structure of a protein from low activity form to high activity form
To make reactants proximal to each other
Intrinsic interaction energy and geometric distortions are involved
ES complex is selectively destabilized

Discuss Question

Answer: Option B. -> To make reactants proximal to each other
Answer: (b).To make reactants proximal to each other

Question 26. The change in absorbance is used as the basis for assaying enzymes using __________

radioisotope method
luminescence method
biosensors
spectrophotometer

Discuss Question

Answer: Option D. -> spectrophotometer
Answer: (d).spectrophotometer

Question 27. Which of the following enzyme is assayed and measured using Spectrophotometer?

Hydrolases
Bacterial luciferase
Phosphofructokinase
Glutamate decarboxylase

Discuss Question

Answer: Option C. -> Phosphofructokinase
Answer: (c).Phosphofructokinase

Question 28. Which of the following is not true for spectrofluorometric method?

The intensity of emitted light is used to study enzyme reaction
Trace impurities in enzyme can stop the emitted radiation
It is based on stoke shift
Hydrolases can be assayed and measured by using this method

Discuss Question

Answer: Option A. -> The intensity of emitted light is used to study enzyme reaction
Answer: (a).The intensity of emitted light is used to study enzyme reaction

Question 29. The increase in wavelength due to absorption in UV region and emission in visible light is known as ____________