Questions and answers for exam Preparation

MCQs

Total Questions : 35 | Page 2 of 4 pages

Question 11. Which of these factors is not true for enzymes while controlling assays?

Extreme high salt concentration cannot be tolerated
pH (6-8) is suitable for maximum activity
Decrease in temperature leads to decreased reaction rates
Increase in substrate concentration leads to increase in rate of reaction

Discuss Question

Answer: Option C. -> Decrease in temperature leads to decreased reaction rates
Answer: (c).Decrease in temperature leads to decreased reaction rates

Question 12. Which of these factors is true for enzymes while controlling assays?

Extreme high salt concentration can be tolerated
pH (6-8) is suitable for maximum activity
Decrease in temperature leads to decreased reaction rates
Decrease in substrate concentration leads to increase in rate of reaction

Discuss Question

Answer: Option B. -> pH (6-8) is suitable for maximum activity
Answer: (b).pH (6-8) is suitable for maximum activity

Question 13. Which of the following is not a step involved in discontinuous assay?

Taking sample from the reaction mixture at known time
Stopping the reaction by inactivating the enzyme
Measure the convenient change in absorbance
Measuring the amount of product formed

Discuss Question

Answer: Option C. -> Measure the convenient change in absorbance
Answer: (c).Measure the convenient change in absorbance

Question 14. Macromolecular crowding does alter the rate and equilibrium constant of enzyme reaction.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 15. In which of the following assays, there is a “stop and sample” procedure?

Continuous assay
Coupled assay
Enzymatic assay technique
Discontinuous assay

Discuss Question

Answer: Option D. -> Discontinuous assay
Answer: (d).Discontinuous assay

Question 16. Which of the following assays, there is a convenient change is absorbance?

Continuous assay
Coupled assay
Enzymatic assay technique
Discontinuous assay

Discuss Question

Answer: Option A. -> Continuous assay
Answer: (a).Continuous assay

Question 17. Which of the following precautions must not be followed while performing assays?

The substrates, buffers etc., should be of high purity
Enzyme preparation should as pure as possible
The probe must be tiny and biocompatible
Stability of the enzyme during the time taken by assay

Discuss Question

Answer: Option C. -> The probe must be tiny and biocompatible
Answer: (c).The probe must be tiny and biocompatible

Question 18. Which of the following is a precaution which must be followed while performing assays?

It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added
The response should be accurate, reproducible, precise, linear and free from electrical noise
The probe must be tiny and biocompatible
It should be cheap, small portable and capable of being used by semi-skilled operators

Discuss Question

Answer: Option A. -> It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added
Answer: (a).It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added

Question 19. The reaction catalyzed by ornithine decarboxylase is an example of which assay?