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12th Grade > Biology

BIOTECHNOLOGY AND ITS APPLICATIONS MCQs

Total Questions : 63 | Page 4 of 7 pages
Question 31. The trigger for activation of toxin of Bacillus thuringiensis is:
  1.    Acidic pH of stomach
  2.    High temperature
  3.    Alkaline pH of gut
  4.    Mechanical action in the insect gut
 Discuss Question
Answer: Option C. -> Alkaline pH of gut
:
C
Bt cotton is produced by introducing an endotoxin producing gene from B. thuringiensis. Once Bt cotton grows, it starts producing these toxins. This toxin enters the bollworm when they feed on cotton leaves. Upon reaching the alkaline environment of the insect’s midgut the toxin is activated and binds to specific receptors present on the lining cells. This causes autodigestion and creates holes in the midgut region leading to the death of the insect.
Question 32. AIDS can be detected by PCR at an early stage as
  1.    it carries out isolation fast
  2.    No other technique is available
  3.    PCR has to be done before hybridization can be done
  4.    It amplifies the genetic material
 Discuss Question
Answer: Option D. -> It amplifies the genetic material
:
D
AIDS can be detected by PCR (polymerase chain reaction) at early stages as it amplifies the genetic material millions of times. Thus the presence of even a tiny number of viruses can give a positive result to the diagnostic test.
Question 33. What are the options for increasing food production?
  1.    agro-chemical based agriculture
  2.    organic agriculture
  3.    genetically engineered crop-based agriculture
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
:
D
Few techniques and technologies were needed to improve the output of food crops to meet the ever growing demand of the human population. The three options that have been used for increasing food production are (i) agro-chemical based agriculture (ii) organic agriculture; and (iii) genetically engineered crop-based agriculture.
Question 34. Who is known as the ‘Indian Father of the Green Revolution’?
 
  1.    Dr. Norman Borlaug
  2.    Dr. MS Swaminathan
  3.    Dr. APJ Abdul Kalam
  4.    Dr. Hargobind Khurana
 Discuss Question
Answer: Option B. -> Dr. MS Swaminathan
:
B
Dr. MS Swaminathan is known as the ‘Indian Father of Green Revolution’ for his leadership and success in introducing and further developing high-yielding varieties of food grains in India. The extensive work done by him and his team in this field helped India become self-sufficient in the production of food grains, especially Wheat.
Question 35. Bioplastic can be made using a simple sugar from a fungus.
  1.    True
  2.    False
 Discuss Question
Answer: Option A. -> True
:
A
A Japanese company called Hayashibara made bioplastic by using simple sugar derived from the fungus Pullularia pullulans. They constructed a polysaccharide called ‘pullulan’ which possessed the physical properties of plastic but was also biodegradable as it was made of only sugars.
Question 36. ‘Dolly’ was created using the udder cell nucleus from __________  and an enucleated egg cell from ____________
  1.    Finn dorset ewe and Scottish blackface ewe
  2.    Scottish blackface ewe and Finn dorset ewe
  3.    Scottish blackface ewe and Finn dorset Ram
  4.    Only from the udder cells of finn dorset ewe
 Discuss Question
Answer: Option A. -> Finn dorset ewe and Scottish blackface ewe
:
A
Dolly was cloned using the process of nuclear transfer. Threefemale sheep were used to produce the clone.The udder cell from a finn Dorset was taken. This is a somatic cell. The nucleus was removed and then inserted into an enucleated egg cell from a scottish blackface ewe. This zygote was allowed to develop into an embryo and then introduced into theuterus of another scottish blackface ewe. Even though the enucleated egg cell and the surrogate mother both were blackface ewes the offspring dolly was born as a Finn Dorset.
Question 37. What are the options for increasing food production?
  1.    agro-chemical based agriculture
  2.    organic agriculture
  3.    genetically engineered crop-based agriculture
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
:
D
Few techniques and technologies were needed to improve the output of food crops to meet the ever growing demand of the human population. The three options that have been used for increasing food production are (i) agro-chemical based agriculture (ii) organic agriculture; and (iii) genetically engineered crop-based agriculture.
Question 38. Which among the four diseases are treated with biological products obtained from transgenic animals?
i. Emphysema   
ii. AIDS    
iii. Cystic fibrosis   
iv. Phenylketonuria
  1.    Only i, ii and iii
  2.    Only ii, iii and iv
  3.    Only i, iii and iv
  4.    Only i, ii and iv
 Discuss Question
Answer: Option C. -> Only i, iii and iv
:
C
Useful biological products are obtained from transgenic animals to treat diseases like Emphysema, Cystic fibrosis and Phenylketonuria. The treatment of AIDS is the use of antivirals that are not obtained from transgenic animals.
Question 39. AIDS can be detected by PCR at an early stage as
  1.    it carries out isolation fast
  2.    No other technique is available
  3.    PCR has to be done before hybridization can be done
  4.    It amplifies the genetic material
 Discuss Question
Answer: Option D. -> It amplifies the genetic material
:
D
AIDS can be detected by PCR (polymerase chain reaction) at early stages as it amplifies the genetic material millions of times. Thus the presence of even a tiny number of viruses can give a positive result to the diagnostic test.
Question 40. The main challenge for the production of insulin using rDNA technique was
  1.    Joining chain A with C peptide chain
  2.    Getting insulin assembled into its mature form
  3.    Removing B peptide chain
  4.    Separating chain A and chain B
 Discuss Question
Answer: Option B. -> Getting insulin assembled into its mature form
:
B
The main challenge for production of insulin using rDNA technique was getting insulin assembled into its mature form. The primitive form of insulin called proinsulin contains the C peptide which is not a part of mature insulin. Therefore the scientists had to make the mature insulin using reverse transcriptase and the mature mRNA of the insulin gene. This method is known as cDNA technology, where complimentary DNA sequene of the mRNA is made and inserted as a gene of interest into the bacteria.

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