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12th Grade > Biology

BIOTECHNOLOGY AND ITS APPLICATIONS MCQs

Total Questions : 63 | Page 3 of 7 pages
Question 21. The phenomenon of introducing exogenous DNA into the genome of plant or animal is called _____.
  1.    Tissue culture
  2.    Transgenesis
  3.    Transfection
  4.    Foreign gene therapy
 Discuss Question
Answer: Option B. -> Transgenesis
:
B
The phenomenon of introducing exogenous DNA or DNA from another organism into the genome of plant or animal is called transgenesis. The foreign DNA introduced in this process is called a transgene and the animal in which it is introduced is called a transgenic animal.
Question 22. The first instance of gene therapy recorded was in relation to which disease?
  1.    Insulin Deficiency 
  2.    Adenosine Deaminase Deficiency
  3.    Asthma
  4.    Lung Cancer
 Discuss Question
Answer: Option B. -> Adenosine Deaminase Deficiency
:
B
The first successful clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency. This enzyme is crucial for the immune system to function.
Question 23. Which of the following is true about gene therapy? 
  1.    A technique used to replace a defective gene with a healthy gene 
  2.    A technique that corrects the defective gene
  3.    A technique used to cure genetic disease
  4.    All of the these 
 Discuss Question
Answer: Option D. -> All of the these 
:
D
Gene therapy is used to treatgenetic disorders resulting due to the presenceof anonfunctional gene copy. In this case, functional genes are inserted into the cells or tissue of a patient and then those cells are delivered to the patient to compensate for the effects of the non-functional gene.
Question 24. During gene insertion using microinjection, transgenes are added to the:
  1.    Sperm
  2.    Egg
  3.    Male pronucleus
  4.    Female pronucleus
 Discuss Question
Answer: Option C. -> Male pronucleus
:
C
The egg and the sperm fuse to form a zygote.The zygote stage is very brief. In the zygote we have the male and female pronucleus.The transgene is added to the male pronucleus as it is bigger. After this, the male and the femal pronuclei fuse and further divide to form the embryo which is then implanted into a surrogate mother to develop further into a full fledged organism.
Question 25. Who is known as the ‘Indian Father of the Green Revolution’?
 
  1.    Dr. Norman Borlaug
  2.    Dr. MS Swaminathan
  3.    Dr. APJ Abdul Kalam
  4.    Dr. Hargobind Khurana
 Discuss Question
Answer: Option B. -> Dr. MS Swaminathan
:
B
Dr. MS Swaminathan is known as the ‘Indian Father of Green Revolution’ for his leadership and success in introducing and further developing high-yielding varieties of food grains in India. The extensive work done by him and his team in this field helped India become self-sufficient in the production of food grains, especially Wheat.
Question 26. which of the following is true about the first transgenic cow Rosie?
  1.    It produced human protein-enriched milk (2.4 grams per litre).
  2.    The milk contained the human alpha-lactalbumin
  3.    the milk produced by Rosie was a more balanced product for human babies than natural cow-milk.
  4.    all of these
 Discuss Question
Answer: Option D. -> all of these
:
D
In 1997 the first transgenic cow Rosie produced human protein-enriched milk (2.4 grams per litre). The milk contained the human protein alpha-lactalbumin and was nutritionally a more balanced product for consumption by human babies than natural cow-milk.
Question 27. The organization set up by Indian government for making decisions regarding the validity of GM research is
  1.    Genetic research committee
  2.    Genetic Engineering Approval Committee
  3.    Genetic engineering safety committee
  4.    All of the above
 Discuss Question
Answer: Option B. -> Genetic Engineering Approval Committee
:
B
The indian government has set up organizations such as the genetic engineering approval committee (GEAC) to make decisions regarding the validity of GM research and safety of introducing GM organisms for public services in India.
Question 28. ‘Dolly’ was created using the udder cell nucleus from __________  and an enucleated egg cell from ____________
  1.    Finn dorset ewe and Scottish blackface ewe
  2.    Scottish blackface ewe and Finn dorset ewe
  3.    Scottish blackface ewe and Finn dorset Ram
  4.    Only from the udder cells of finn dorset ewe
 Discuss Question
Answer: Option A. -> Finn dorset ewe and Scottish blackface ewe
:
A
Dolly was cloned using the process of nuclear transfer. Threefemale sheep were used to produce the clone.The udder cell from a finn Dorset was taken. This is a somatic cell. The nucleus was removed and then inserted into an enucleated egg cell from a scottish blackface ewe. This zygote was allowed to develop into an embryo and then introduced into theuterus of another scottish blackface ewe. Even though the enucleated egg cell and the surrogate mother both were blackface ewes the offspring dolly was born as a Finn Dorset.
Question 29. Which of the following cannot be used to isolate insulin used by diabetic patients?
  1.    Pig
  2.    Calves
  3.    Viruses 
  4.    Transgenic bacteria
 Discuss Question
Answer: Option C. -> Viruses 
:
C
In 1921, Banting and Best first isolated insulin from fetal calves. Later, pigs and cows became the two major sources of insulin. This was at the time when genetic engineering was yet to be developed and insulin for humans was extracted from animal sources. This was extremely expensive and the quantity was very low.
Now, genetic engineering has enabled us to produce mammalian insulin from GM bacteria.
Viruses cannot be grown in synthetic medium and
hijack the host splicing machinery to process their RNA segments hence they cannot be used for the production of biological products.
Question 30. Which among the four diseases are treated with biological products obtained from transgenic animals?
i. Emphysema   
ii. AIDS    
iii. Cystic fibrosis   
iv. Phenylketonuria
  1.    Only i, ii and iii
  2.    Only ii, iii and iv
  3.    Only i, iii and iv
  4.    Only i, ii and iv
 Discuss Question
Answer: Option C. -> Only i, iii and iv
:
C
Useful biological products are obtained from transgenic animals to treat diseases like Emphysema, Cystic fibrosis and Phenylketonuria. The treatment of AIDS is the use of antivirals that are not obtained from transgenic animals.

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