Which of the following is not a step in producing penicillin -G-amidase?

Question

Options:

A . Cleavage by Hind III

B . Replacement of asparagine residue with serine present in the native enzyme

C . Cleaved fragments cloned into a cosmid vector

D . Penicillin-G-amidase gene transferred on to pBR322 plasmids

Answer: Option B
Answer: (b).Replacement of asparagine residue with serine present in the native enzyme

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