Which of the following is not a step involved in site directed mutagenesis using

Question

Which of the following is not a step involved in site directed mutagenesis using a hypothetical example?

Options:

A . Derive the primary structure of the enzyme from the DNA sequence

B . Identification of mutant DNA by hybridization with radioactively labelled oligonucleotides

C . Cleavage by Hind III

D . Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme

Answer: Option C
Answer: (c).Cleavage by Hind III

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