A DNA Microarray or ‘gene-chip’ consists of: Options: A . &nbspA single-stranded DNA sample attached to the microarray surface and fluorescent labelled RNA probes B . &nbspAn RNA probe attached to the microarray surface and fluorescent labelled DNA samples C . &nbspAn RNA sample attached to the microarray surface and fluorescent labelled RNA probes D . &nbspA single-stranded DNA probe attached to the microarray surface and fluorescent labelled single-stranded cDNA samples

A DNA Microarray or ‘gene-chip’ consists of:

Question

A DNA Microarray or ‘gene-chip’ consists of:

Options:

A . A single-stranded DNA sample attached to the microarray surface and fluorescent labelled RNA probes

B . An RNA probe attached to the microarray surface and fluorescent labelled DNA samples

C . An RNA sample attached to the microarray surface and fluorescent labelled RNA probes

D . A single-stranded DNA probe attached to the microarray surface and fluorescent labelled single-stranded cDNA samples

Answer: Option D
:
D
A gene chip or a microarray consists of synthesized probes of the DNA sequence of the gene to be studied. RNA of the gene whose expression we are studying is isolated and converted to fluorescently labelled cDNA known as the gene sample. The hybridization of the sample with the probe produces fluorescence which is picked up by a specialized CCD camera. The more the fluorescence, more is the activity of the gene in the cell and vice versa.

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