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MCQs

Total Questions : 42 | Page 2 of 5 pages
Question 11. . Maximum amount of foreign DNA introduced into SV 40 viruses is
  1.    10 kb
  2.    5 kb
  3.    2.5 kb
  4.    1 kb
 Discuss Question
Answer: Option C. -> 2.5 kb
Question 12. . Which of the following is/are commonly used promoter of S. cerevisiae?
  1.    Galactokinase (GAL 1)
  2.    Alcohol dehydrogenase II (ADH 2)
  3.    Phosphoglycerate kinase (PGK)
  4.    All of the above
 Discuss Question
Answer: Option D. -> All of the above
Question 13. . Recombinant SV40 viruses introduce the foreign DNA into cells
  1.    with the DNA mediated transfection
  2.    without the DNA mediated transfection
  3.    with the RNA mediated transfection
  4.    without the RNA mediated transfection
 Discuss Question
Answer: Option B. -> without the DNA mediated transfection
Question 14. . Which one of the following is the most stable vector?
  1.    YEp
  2.    YRp
  3.    YAC
  4.    YIp
 Discuss Question
Answer: Option C. -> YAC
Question 15. . SV 40 vectors, in which early region replacements are carried out, require
  1.    COS cell line
  2.    SV 40 helper virus
  3.    pBR 322 vector
  4.    none of these
 Discuss Question
Answer: Option A. -> COS cell line
Question 16. . Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
  1.    Allows transformation by using crossing over
  2.    Has a origin of replication and rep genes
  3.    Carries autonomously replicating sequence
  4.    Carries centromere and telomere sequences
 Discuss Question
Answer: Option B. -> Has a origin of replication and rep genes
Question 17. . Cloning genes in the yeast offer which of following advantage(s)?
  1.    Over production of proteins of commercial value
  2.    Ability to glycosylate proteins during secretion
  3.    Ability to clone large piece of DNA
  4.    All of the above
 Discuss Question
Answer: Option D. -> All of the above
Question 18. . Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
  1.    Contains centromeric sequences
  2.    Segregates during meiosis and mitosis
  3.    Functions as true chromosome
  4.    All of the above
 Discuss Question
Answer: Option D. -> All of the above
Question 19. . Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of
  1.    Vaccinia virus
  2.    Baculo virus
  3.    Papilloma virus
  4.    Retrovirus
 Discuss Question
Answer: Option B. -> Baculo virus
Question 20. . Cos cell line is
  1.    cohesive end site of phage λ
  2.    derivative of permissive CV-1 monkey cell line
  3.    cohesive initial site of phage λ
  4.    derivative of non-permissive CV-1 monkey cell line
 Discuss Question
Answer: Option B. -> derivative of permissive CV-1 monkey cell line

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