Questions and answers for exam Preparation

MCQs

Total Questions : 301 | Page 6 of 31 pages

Question 51. Which part of the amino acid gives it uniqueness?

Amino group
Carboxyl group
Side chain
None of the mentioned

Discuss Question

Answer: Option C. -> Side chain
Answer: (c).Side chain

Question 52. The salt which produces salting out effect during extraction of proteins is?

NH₄SO₄
(NH₄)₂SO₄
(NH₄)₃SO₄
NaCl

Discuss Question

Answer: Option B. -> (NH₄)₂SO₄
Answer: (b).(NH₄)₂SO₄

Question 53. What are the following is not a factor responsible for the denaturation of proteins?

pH change
Organic solvents
Heat
Charge

Discuss Question

Answer: Option D. -> Charge
Answer: (d).Charge

Question 54. Which of the following information is responsible to specify the three-dimensional shape of a protein?

The protein’s peptide bond
The protein’s amino acid sequence
The protein’s interaction with other polypeptides
The protein’s interaction with molecular chaperons

Discuss Question

Answer: Option B. -> The protein’s amino acid sequence
Answer: (b).The protein’s amino acid sequence

Question 55. Unfolding of a protein can be termed as _________

Renaturation
Denaturation
Oxidation
Reduction

Discuss Question

Answer: Option B. -> Denaturation
Answer: (b).Denaturation

Question 56. Mobile phase can be ___________

Only solid
Only gas
Solid or liquid
Liquid or gas

Discuss Question

Answer: Option D. -> Liquid or gas
Answer: (d).Liquid or gas

Question 57. Which of the following statements is true about size-exclusion chromatography?

During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first
During the separation of a mixture of proteins, protein with largest molecular weight is eluted first
During the separation of a mixture of proteins, protein with largest molecular weight is eluted last
During the separation of a mixture of proteins, protein with largest molecular weight flow around the beads

Discuss Question

Answer: Option B. -> During the separation of a mixture of proteins, protein with largest molecular weight is eluted first
Answer: (b).During the separation of a mixture of proteins, protein with largest molecular weight is eluted first

Question 58. Which of the following statements about column chromatography is correct?

Resolution increases as the length of the column increases
Mobile phase is a porous solid material with appropriate chemical properties held in the column
Stationary phase is a buffered solution that percolates through mobile phase
Large proteins emerge from the column sooner than small ones

Discuss Question

Answer: Option A. -> Resolution increases as the length of the column increases
Answer: (a).Resolution increases as the length of the column increases

Question 59. Which of the following statements is true about affinity chromatography?

During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first
During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last
During the separation of a mixture of proteins, the protein which binds to ligand is eluted first
Unwanted proteins are eluted by ligand solution

Discuss Question

Answer: Option A. -> During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first
Answer: (a).During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first

Question 60. The pattern on paper in chromatography is called __________