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Total Questions : 46 | Page 4 of 5 pages
Question 31. . The DNA sequences of rRNA genes can be used to
  1.    assess relatedness of eukaryotes but not prokaryotes
  2.    obtain a tentative identification of a new microorganism
  3.    predict the pulsed field gel pattern of a microorganism
  4.    predict whether a bacteria will be gram-positive or gram-negative
 Discuss Question
Answer: Option B. -> obtain a tentative identification of a new microorganism
Question 32. . When mutations involve vital functions so that the mutants are nonviable, it is a
  1.    nonsense mutation
  2.    lethal mutation
  3.    transversion
  4.    none of these
 Discuss Question
Answer: Option B. -> lethal mutation
Question 33. . Which of the following mutation affects only a single nucleotide?
  1.    Aerial mutation
  2.    Site mutation
  3.    Point mutation
  4.    Regional mutation
 Discuss Question
Answer: Option C. -> Point mutation
Question 34. . A mutagen is defined as
  1.    an enzyme that repairs mutations
  2.    a chemical or physical agent that induces mutations
  3.    an inhibitor of gene modification
  4.    a molecule which stabilizes DNA thus prevents mutations from occurring
 Discuss Question
Answer: Option B. -> a chemical or physical agent that induces mutations
Question 35. . A nonsense mutation introduced into the DNA sequence of a gene may
  1.    cause premature termination of the mRNA
  2.    shorten the length of the protein encoded by the gene
  3.    have no effect on the transcript or protein made
  4.    cause a shift in reading frame
 Discuss Question
Answer: Option B. -> shorten the length of the protein encoded by the gene
Question 36. . The promoter on an expression vector used to overproduce proteins in bacteria is
  1.    a bacterial promoter
  2.    expressed both in bacteria and mammalian cells
  3.    not a regulated promoter
  4.    the natural promoter of the gene being cloned
 Discuss Question
Answer: Option A. -> a bacterial promoter
Question 37. . Which of these mechanisms for thymine dimers repair lead to mutations?
  1.    Excision repair
  2.    Photoreactivation
  3.    SOS repair
  4.    Never leads to mutation
 Discuss Question
Answer: Option C. -> SOS repair
Question 38. . Frame shift mutation may occur as a result of
  1.    formation of a thymine-dimer
  2.    deamination of cytosine to uracil
  3.    conversion of guanine to xanthine
  4.    none of the above
 Discuss Question
Answer: Option D. -> none of the above
Question 39. . Thymine dimers are directly repaired with the help of visible light by process known as
  1.    phosphorylation
  2.    excision repair
  3.    photosynthesis
  4.    photoreactivation
 Discuss Question
Answer: Option D. -> photoreactivation
Question 40. . Differentiation of four different bases in automated sequencing systems is that each base has
  1.    different radioactive tag
  2.    cytosine at start
  3.    unique antibody bound to it
  4.    distinctive fluorescent tag
 Discuss Question
Answer: Option D. -> distinctive fluorescent tag

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