Questions and answers for exam Preparation

MCQs

Total Questions : 54 | Page 1 of 6 pages

Question 1. The support material for immobilization of cells of Bacillus subtilis is

ion exchange resins
gelatin
Anthracite
agarose and carbodiimide

Discuss Question

Answer: Option D. -> agarose and carbodiimide
Answer: (d).agarose and carbodiimide

Question 2. Which of the following is not a physical method of immobilization?

Adsorption
Entrapment
Micro encapsulation
None of these

Discuss Question

Answer: Option D. -> None of these
Answer: (d).None of these

Question 3. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

copolymerization or microencapsulation
crosslinking using multifunctional reagents
adsorption
all of the above

Discuss Question

Answer: Option A. -> copolymerization or microencapsulation
Answer: (a).copolymerization or microencapsulation

Question 4. The covalent attachment of enzyme molecules is via

nonessential amino acids residues to water insoluble, functional supports
essential amino acids residues to water insoluble, functional supports
nonessential amino acids residues to water soluble, functional supports
essential amino acids residues to water soluble, functional supports

Discuss Question

Answer: Option A. -> nonessential amino acids residues to water insoluble, functional supports
Answer: (a).nonessential amino acids residues to water insoluble, functional supports

Question 5. To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as

reaction rate/rate if not slowed by diffusion
rate if not slowed by diffusion/ reaction rate
actual reaction rate/ rate if not slowed by diffusion
rate if not slowed by diffusion/ actual reaction rate

Discuss Question

Answer: Option C. -> actual reaction rate/ rate if not slowed by diffusion
Answer: (c).actual reaction rate/ rate if not slowed by diffusion

Question 6. Which of the following is considered as a disadvantage to the adsorption method of immobilization?

It is possible to separate and purify the enzymes while being immobilized
The enzymes are not usually deactivated by adsorption
The adsorption is a reversible process
State of immobilization is very sensitive to solution pH, ionic strength and temperature

Discuss Question

Answer: Option D. -> State of immobilization is very sensitive to solution pH, ionic strength and temperature
Answer: (d).State of immobilization is very sensitive to solution pH, ionic strength and temperature

Question 7. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

equal to that of substrate consumption
more than that of substrate consumption
lesser than that of substrate consumption
is nothing to do with the substrate consumption

Discuss Question

Answer: Option A. -> equal to that of substrate consumption
Answer: (a).equal to that of substrate consumption

Question 8. The immobilized technique involving chemical method is

covalent bond formation dependent
non-covalent bond formation dependent
both (a) and (b)
ionic bond formation dependent

Discuss Question

Answer: Option A. -> covalent bond formation dependent
Answer: (a).covalent bond formation dependent

Question 9. Damkohler number(NDa) is

the ratio of the maximum reaction rate to the maximum mass transfer rate
the ratio of the minimum reaction rate to the maximum mass transfer rate
the ratio of the maximum reaction rate to the minimum mass transfer rate
the ratio of the minimum reaction rate to the minimum mass transfer rate

Discuss Question

Answer: Option A. -> the ratio of the maximum reaction rate to the maximum mass transfer rate
Answer: (a).the ratio of the maximum reaction rate to the maximum mass transfer rate

Question 10. For a steady state condition, the change of substrate concentration (dCs/dt) is