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MCQs

Total Questions : 232 | Page 1 of 24 pages
Question 1. Treatment with exonuclease leads to removal of ____________
  1.    remnants of chromosomal DNA
  2.    RNase
  3.    plasmid DNA which is circularized
  4.    proteins
 Discuss Question
Answer: Option A. -> remnants of chromosomal DNA
Answer: (a).remnants of chromosomal DNA
Question 2. Nucleic acid precipitated constitutes of ____________
  1.    plasmid DNA
  2.    plasmid DNA, along with RNA and chromosomal DNA
  3.    rna alone
  4.    chromosomal DNA only
 Discuss Question
Answer: Option B. -> plasmid DNA, along with RNA and chromosomal DNA
Answer: (b).plasmid DNA, along with RNA and chromosomal DNA
Question 3. Adsorption onto a solid phase support followed by elution is used as an alternative for separation of which component?
  1.    chromosomal DNA
  2.    plasmid DNA
  3.    RNA alone
  4.    other impurities
 Discuss Question
Answer: Option B. -> plasmid DNA
Answer: (b).plasmid DNA
Question 4. Which of the following components bind to the solid column made of silica, under high salt concentration?
  1.    Proteins
  2.    Polysaccharides
  3.    Both proteins and polysaccharides
  4.    Plasmid DNA
 Discuss Question
Answer: Option D. -> Plasmid DNA
Answer: (d).Plasmid DNA
Question 5. Purification of DNA by using silica derivatized groups by DEAE is termed as ____________
  1.    ion exchange resin based method
  2.    silica based purification
  3.    atom based resin exchange method
  4.    packed bed purification
 Discuss Question
Answer: Option A. -> ion exchange resin based method
Answer: (a).ion exchange resin based method
Question 6. Which of the following statements is correct regarding S1 nuclease?
  1.    It acts on double stranded DNA
  2.    It acts on single stranded DNA
  3.    It acts on both types of strands
  4.    It is obtained from E. coli
 Discuss Question
Answer: Option B. -> It acts on single stranded DNA
Answer: (b).It acts on single stranded DNA
Question 7. What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?
  1.    The molecule is shortened only from 3’ end
  2.    The molecule is shortened only from 5’ end
  3.    The molecule is shortened from both the ends
  4.    Only Exonuclease acts and S1 doesn’t acts
 Discuss Question
Answer: Option C. -> The molecule is shortened from both the ends
Answer: (c).The molecule is shortened from both the ends
Question 8. The extent of deletions can be manipulated by controlling which of them?
  1.    Time of incubation only
  2.    Amount of nuclease added
  3.    They both have a role to play in the extent of the deletion
  4.    The amount of deletion which can be carried out for a particular amount of DNA is fixed regardless of the amount of nuclease and incubation time
 Discuss Question
Answer: Option C. -> They both have a role to play in the extent of the deletion
Answer: (c).They both have a role to play in the extent of the deletion
Question 9. Bal31 is also an enzyme which is used. Which of the following statements hold true in its context?
  1.    It is having only 3’-5’ exonuclease activity and no endonuclease activity
  2.    It is having only an endonuclease activity
  3.    It is having 5’-3’ exonuclease activity
  4.    It leads to shortening from both the ends
 Discuss Question
Answer: Option D. -> It leads to shortening from both the ends
Answer: (d).It leads to shortening from both the ends
Question 10. How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?
  1.    By using Phosphorothioate nucleotide analogue
  2.    By making both the ends double stranded in nature
  3.    By labelling one end with a radioactive compound
  4.    By increasing the time of exposure of the DNA molecule to the enzyme
 Discuss Question
Answer: Option A. -> By using Phosphorothioate nucleotide analogue
Answer: (a).By using Phosphorothioate nucleotide analogue

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