How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends? Options: A . &nbspBy using Phosphorothioate nucleotide analogue B . &nbspBy making both the ends double stranded in nature C . &nbspBy labelling one end with a radioactive compound D . &nbspBy increasing the time of exposure of the DNA molecule to the enzyme

How can one end be protected from the action of Exonuclease III, so that the mol

Question

How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?

Options:

A . By using Phosphorothioate nucleotide analogue

B . By making both the ends double stranded in nature

C . By labelling one end with a radioactive compound

D . By increasing the time of exposure of the DNA molecule to the enzyme

Answer: Option A
Answer: (a).By using Phosphorothioate nucleotide analogue

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Question 1. If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried o....is observed, which type of reaction is being carried out?

Question 2. Enzyme commonly used for carrying out ligation reaction is ____________....

Question 3. What are the effects of increasing concentration of reaction components?....

Question 4. What is the kinetics of the intramolecular and intermolecular ligation reactions?....

Question 5. Which of the following statements is correct with respect to T4 DNA ligase?....

Question 6. If blunt ended ligations are to be carried out. Which of the following enzymes can be used?....

Question 7. Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of....Which of the steps is involved in the ligation mechanism?

Question 8. E. coli DNA ligase doesn’t require a cofactor.....

Question 9. Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?....

Question 10. Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________....portion to another. The enzyme carrying out this is ____________

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