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MCQs

Total Questions : 232 | Page 3 of 24 pages
Question 21. Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________
  1.    inserting the DNA into the cells via an electric shock
  2.    increased efficiency than both natural and chemical methods
  3.    causing the least amount of damage in comparison to other methods
  4.    decreased efficiency than both natural and chemical methods
 Discuss Question
Answer: Option A. -> inserting the DNA into the cells via an electric shock
Answer: (a).inserting the DNA into the cells via an electric shock
Question 22. Transformation carried out using a particle gun is known as biolistic transformation. It falls under which category of transformation?
  1.    Physical
  2.    Chemical
  3.    Electroporation
  4.    Natural
 Discuss Question
Answer: Option A. -> Physical
Answer: (a).Physical
Question 23. How many methods are there for obtaining the plasmid DNA from the bacteria?
  1.    1
  2.    2
  3.    3
  4.    4
 Discuss Question
Answer: Option B. -> 2
Answer: (b).2
Question 24. Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?
  1.    Cell lysis
  2.    Removal of proteins
  3.    Removal of chromosomal DNA
  4.    Dissolving plasmid in water
 Discuss Question
Answer: Option D. -> Dissolving plasmid in water
Answer: (d).Dissolving plasmid in water
Question 25. The particle gun method consists of which of the following steps?
  1.    The DNA of interest is absorbed onto microprojectile beads
  2.    These beads are often made of aluminium
  3.    The explosion in a gun propels a macroprojectile forward which in turn propels microprojectile beads
  4.    The macroprojectiles and microprojectiles both cross the perforated plate and hit the target tissue behind it
 Discuss Question
Answer: Option C. -> The explosion in a gun propels a macroprojectile forward which in turn propels microprojectile beads
Answer: (c).The explosion in a gun propels a macroprojectile forward which in turn propels microprojectile beads
Question 26. The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 27. Chromosomal or genomic DNA is separated by ____________
  1.    Sedimentation
  2.    Dissolution in water
  3.    Centrifugation
  4.    Distillation
 Discuss Question
Answer: Option C. -> Centrifugation
Answer: (c).Centrifugation
Question 28. Proteins can be removed via treatment by?
  1.    Phenol and chloroform treatment
  2.    Treatment with sodium hydroxide
  3.    Chloroform treatment alone
  4.    Centrifuging
 Discuss Question
Answer: Option A. -> Phenol and chloroform treatment
Answer: (a).Phenol and chloroform treatment
Question 29. Cell lysis is carried out by which substance?
  1.    Lysozyme and detergents
  2.    Water
  3.    Sugar solution
  4.    Suphuric Acid
 Discuss Question
Answer: Option A. -> Lysozyme and detergents
Answer: (a).Lysozyme and detergents
Question 30. Gel electrophoresis separates nucleic acid molecules based on ____________
  1.    charge on molecules
  2.    size of the molecules
  3.    nature of the molecules i.e. whether DNA or RNA
  4.    chemical properties of the nucleic acids
 Discuss Question
Answer: Option B. -> size of the molecules
Answer: (b).size of the molecules

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