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Total Questions : 238 | Page 5 of 24 pages
Question 41. SV 40 vectors, in which early region replacements are carried out, require
  1.    COS cell line
  2.    SV 40 helper virus
  3.    pBR 322 vector
  4.    none of these
 Discuss Question
Answer: Option A. -> COS cell line
Answer: (a).COS cell line
Question 42. Cos cell line is
  1.    cohesive end site of phage λ
  2.    derivative of permissive CV-1 monkey cell line
  3.    cohesive initial site of phage λ
  4.    derivative of non-permissive CV-1 monkey cell line
 Discuss Question
Answer: Option B. -> derivative of permissive CV-1 monkey cell line
Answer: (b).derivative of permissive CV-1 monkey cell line
Question 43. Moloney-Mu-leukemia virus is an example of
  1.    Vaccinia virus
  2.    Baculo virus
  3.    Retro virus
  4.    none of these
 Discuss Question
Answer: Option C. -> Retro virus
Answer: (c).Retro virus
Question 44. Lytic infection of SV 40 does not occur in
  1.    permissive cells
  2.    non permissive cells
  3.    both (a) and (b)
  4.    none of these
 Discuss Question
Answer: Option B. -> non permissive cells
Answer: (b).non permissive cells
Question 45. Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
  1.    Allows transformation by using crossing over
  2.    Has a origin of replication and rep genes
  3.    Carries autonomously replicating sequence
  4.    Carries centromere and telomere sequences
 Discuss Question
Answer: Option A. -> Allows transformation by using crossing over
Answer: (a).Allows transformation by using crossing over
Question 46. Yeast episomal plasmids (YEp) were constructed using
  1.    2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
  2.    2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
  3.    12μm yeast plasmid and E coli vector pMB 9
  4.    12μm yeast plasmid and E coli vector pBR 322
 Discuss Question
Answer: Option A. -> 2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
Answer: (a).2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
Question 47. Maximum insert size in case of adenovirus vectors is
  1.    6-8 kb
  2.    4-6 kb
  3.    10-12 kb
  4.    20 kb
 Discuss Question
Answer: Option A. -> 6-8 kb
Answer: (a).6-8 kb
Question 48. YAC vectors can be used to obtain artificial chromosomes because it carries
  1.    centromere
  2.    telomere
  3.    autonomously replicating sequence
  4.    all of these
 Discuss Question
Answer: Option D. -> all of these
Answer: (d).all of these
Question 49. SV 40 vectors, in which late region replacements are done, require
  1.    COS cell line
  2.    SV 40 helper virus
  3.    pBR 322 vector
  4.    none of these
 Discuss Question
Answer: Option B. -> SV 40 helper virus
Answer: (b).SV 40 helper virus
Question 50. Which of the following(s) is/are the most commonly used markers in yeast?
  1.    His 3
  2.    Ura 3
  3.    Trp 1
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Answer: (d).All of these

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