Questions and answers for exam Preparation

MCQs

Total Questions : 238 | Page 6 of 24 pages

Question 51. Which of the following are produced in high copy number?

YEp
YRp
Both (a) and (b)
YCp

Discuss Question

Answer: Option C. -> Both (a) and (b)
Answer: (c).Both (a) and (b)

Question 52. Which of the following viruses are used as vectors for transferring genes to animal cells?

SV 40 virus
Bovine papilloma virus (BVP)
Retroviruses
All of these

Discuss Question

Answer: Option D. -> All of these
Answer: (d).All of these

Question 53. Yeast Replicating plasmids (YRp) carry

centromere
telomere
autonomously replicating sequence
both (a) and (c)

Discuss Question

Answer: Option C. -> autonomously replicating sequence
Answer: (c).autonomously replicating sequence

Question 54. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

transcriptional regulators and are essential for viral replication
not necessary for viral replication
coding for virus coat protein
important for packaging of viral DNA into viral head

Discuss Question

Answer: Option A. -> transcriptional regulators and are essential for viral replication
Answer: (a).transcriptional regulators and are essential for viral replication

Question 55. Retroviruses have advantage for being used as vector for animal cells because

they cover a wide host range
infection does not lead to cell death, infected cells produce virus over an indefinite period
viral gene expression is driven by strong promoters
all of the above

Discuss Question

Answer: Option D. -> all of the above
Answer: (d).all of the above

Question 56. Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
all of the above

Discuss Question

Answer: Option D. -> all of the above
Answer: (d).all of the above

Question 57. Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only
integrated only
either episomally or integrated at high copy number
either episomally or integrated at low copy number

Discuss Question

Answer: Option C. -> either episomally or integrated at high copy number
Answer: (c).either episomally or integrated at high copy number

Question 58. The major problem associated with the isolation of free cells and cell aggregates from organs is that of

releasing the cells from their supporting matrix
inhibiting the cells from their supporting matrix
disintegrating the cells from their supporting matrix
none of the above

Discuss Question

Answer: Option A. -> releasing the cells from their supporting matrix
Answer: (a).releasing the cells from their supporting matrix

Question 59. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called