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MCQs

Total Questions : 238 | Page 21 of 24 pages
Question 201.  Disaggregating of cells can be achieved by
  1.    physical disruption
  2.    enzymatic digestion
  3.    treating with chelating agents
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
Question 202.  Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
  1.    150-300
  2.    280-360
  3.    300-325
  4.    360-400
 Discuss Question
Answer: Option B. -> 280-360
Question 203.  Toxicity due to accumulation of ammonia can be overcome
  1.    by substituting glutamine by glutamate
  2.    by controlled addition of glutamine at low level
  3.    by removal of ammonia or ammonium from culture medium
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
Question 204.  Accumulation of lactate leads to
  1.    increase in pH
  2.    no change in pH
  3.    reduction in the pH of culture hence loss of cell viability
  4.    no loss of cell viability
 Discuss Question
Answer: Option C. -> reduction in the pH of culture hence loss of cell viability
Question 205.  According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
  1.    6 amino acids
  2.    8 amino acids
  3.    10 amino acids
  4.    12 amino acids
 Discuss Question
Answer: Option D. -> 12 amino acids
Question 206.  Excess CO2 suppress cell growth and productivity by
  1.    inhibiting respiration
  2.    altering intracellular pH by diffusing across cell membrane
  3.    both (a) and (b)
  4.    altering pH of the medium
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 207.  Which of the following is incorrect?
  1.    Established cell lines (ECL) have short doubling time
  2.    ECL are invariably aneuploid
  3.    ECL grow in higher density
  4.    ECL do not show much evidence of spatial orientation
 Discuss Question
Answer: Option C. -> ECL grow in higher density
Question 208. . What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
  1.    They act as growth promoters
  2.    They act as growth inhibitors
  3.    Have no effect on cells
  4.    Lactate helps in the growth while ammonium inhibits the growth
 Discuss Question
Answer: Option D. -> Lactate helps in the growth while ammonium inhibits the growth
Question 209. . To prevent the accumulation of lactate
  1.    low glutamine concentration is required
  2.    high glutamine concentration is required
  3.    low glucose concentration is required
  4.    high glucose concentration is required
 Discuss Question
Answer: Option C. -> low glucose concentration is required
Question 210. . Which of the following is correct?
  1.    ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
  2.    ECL and PCL can be established in suspension cultures
  3.    PCL can be established in suspension cultures
  4.    none of the above
 Discuss Question
Answer: Option A. -> ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)

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