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Question
Which of the following is untrue regarding the classic yeast two-hybrid method?
Options:
A .  It is used for the detection of Protein interactions
B .  Method that relies on the interaction of “bait” and “prey” proteins in molecular constructs in yeast
C .  DNA-binding domain and a trans-activation domain don’t necessarily interact
D .  In this strategy, a two-domain transcriptional activator is employed as a helper for determining protein–protein interactions
Answer: Option C
Answer: (c).DNA-binding domain and a trans-activation domain don’t necessarily interact

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