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Question
Touch-down PCR is another modification. Its characteristics include:
Options:
A .  Lowering the temperature for primer annealing
B .  Primer annealing is done at higher temperatures initially
C .  The temperature is abruptly reduced in the second cycle
D .  In earlier cycles less stringent conditions are there and in later cycles, more stringent conditions are there
Answer: Option B
Answer: (b).Primer annealing is done at higher temperatures initially

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