Questions and answers for exam Preparation

MCQs

Total Questions : 71 | Page 6 of 8 pages

Question 51. If linkers are combined with other features such as a selectable marker, it is called as __________

cassette
modified linker
adaptors
induced linker

Discuss Question

Answer: Option A. -> cassette
Answer: (a).cassette

Question 52. CcdB gene is used at times. Choose the correct statement with respect to this gene.

It is a control of cell birth gene
It is activated in complex vectors to increase the fraction of recombinants
If intramolecular ligation takes place this protein is released
It is often regarded as control of cell death and birth gene

Discuss Question

Answer: Option B. -> It is activated in complex vectors to increase the fraction of recombinants
Answer: (b).It is activated in complex vectors to increase the fraction of recombinants

Question 53. When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

forced cloning
orientation cloning
correct cloning
restricted cloning

Discuss Question

Answer: Option A. -> forced cloning
Answer: (a).forced cloning

Question 54. What happens if insert DNA is cut with two different restriction enzymes at the ends?

It is difficult to insert the fragment
The insert can be ligated in any orientation
The insert can be ligated in one orientation only
The amount of product increases

Discuss Question

Answer: Option C. -> The insert can be ligated in one orientation only
Answer: (c).The insert can be ligated in one orientation only

Question 55. What is the disadvantage of amplification of using PCR over natural cloning?

In PCR, there is no proof reading activity
In PCR, small fragments can’t be amplified
There is an A incorporated in PCR products, which makes cloning difficult
PCR takes more time as compared to natural cloning

Discuss Question

Answer: Option A. -> In PCR, there is no proof reading activity
Answer: (a).In PCR, there is no proof reading activity

Question 56. TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?

It is based on the fact that a T residue is incorporated at the end of the PCR product
‘A’ residue is incorporated into the end of the vector
It gives a low yield
It is preferred over blunt end ligation

Discuss Question

Answer: Option D. -> It is preferred over blunt end ligation
Answer: (d).It is preferred over blunt end ligation

Question 57. Which of the following characteristic is not present in a plasmid on a general basis?

Multiple cloning site (MCS)
Origin of replication (ori)
Antibiotic resistance gene
Beta galactose genes

Discuss Question

Answer: Option D. -> Beta galactose genes
Answer: (d).Beta galactose genes

Question 58. Bla is a gene, which is incorrect for it?

It is an antibiotic resistance genes
Antibiotic acts by blocking the cross-linking of the bacterial cell wall and thus leading to lysing of cells
It encodes beta lactamase enzyme
The beta lactam ring is activated

Discuss Question

Answer: Option D. -> The beta lactam ring is activated
Answer: (d).The beta lactam ring is activated

Question 59. Which of the following orientation is not propagated to later stages?