Questions and answers for exam Preparation

MCQs

Total Questions : 71 | Page 2 of 8 pages

Question 11. It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using __________

multiple cloning site
origin of replication
high copy number
selectable marker

Discuss Question

Answer: Option D. -> selectable marker
Answer: (d).selectable marker

Question 12. If a restriction site is 6 nucleotides long, what are the chances of finding it in a vector?

Once every 46 base pairs
Once every 64 base pairs
Once every 24 base pairs
Once every 16 base pairs

Discuss Question

Answer: Option A. -> Once every 46 base pairs
Answer: (a).Once every 46 base pairs

Question 13. Multiple cloning site (MCS) is defined as __________

site within the plasmid which contains a site for many restriction enzymes
site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid
as the site containing many sites for only one restriction enzyme
cloning many inserts together

Discuss Question

Answer: Option B. -> site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid
Answer: (b).site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid

Question 14. Size of the vector is related to having a suitable single restriction site.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 15. Which of the statement is true for pBR322?

It contains only an ampicillin resistance gene
It contains both ampicillin resistant and tetracycline resistant gene
The cloning site is present only in the ampicillin resistant gene
It is a natural vector

Discuss Question

Answer: Option B. -> It contains both ampicillin resistant and tetracycline resistant gene
Answer: (b).It contains both ampicillin resistant and tetracycline resistant gene

Question 16. If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?

The insert is not present in any of the gene
The insert is present in ampicillin gene but not in chloramphenicol gene
The insert is present in choramphenicol gene but not in ampicillin gene
The insert is present between both of the genes

Discuss Question

Answer: Option C. -> The insert is present in choramphenicol gene but not in ampicillin gene
Answer: (c).The insert is present in choramphenicol gene but not in ampicillin gene

Question 17. If high copy number is there, the replication is called as ______ and if low copy number is there the replication is called as ______

stringent, relaxed
relaxed, stringent
relaxed, relaxed
stringent, stringent

Discuss Question

Answer: Option A. -> stringent, relaxed
Answer: (a).stringent, relaxed

Question 18. The phenomenon of not allowing the recombinants to escape into the environment is termed as __________

blocking
termination
disablement
inactivation

Discuss Question

Answer: Option C. -> disablement
Answer: (c).disablement

Question 19. Chloramphenicol amplification is carried out in various plasmids. Which of the statement is incorrect for it?

It is used for increasing copy number in plasmids containing pMB1 origin
Chloramphenicol promotes bacterial protein synthesis
The protein synthesis is responsible for chromosomal DNA replication
Chromosomal DNA replication is related to cell division

Discuss Question

Answer: Option B. -> Chloramphenicol promotes bacterial protein synthesis
Answer: (b).Chloramphenicol promotes bacterial protein synthesis

Question 20. How will we choose which transformation method is to be used?

It depends on the available conditions for the reaction to take place
It depends on the efficiency which is required to be obtained
All the transformation methods can be used equivalently
Each reaction has been assigned a particular transformation method

Discuss Question

Answer: Option B. -> It depends on the efficiency which is required to be obtained
Answer: (b).It depends on the efficiency which is required to be obtained