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MCQs

Total Questions : 62 | Page 6 of 7 pages
Question 51. A short molecule containing 2-20 nucleotide is
  1.    plasmid
  2.    oligonucleotide
  3.    vector
  4.    mononucleotide
 Discuss Question
Answer: Option B. -> oligonucleotide
Question 52. Which of the following vector can maintain the largest fragment of foreign DNA?
  1.    Cosmid
  2.    Plasmid
  3.    Phage
  4.    YAC
 Discuss Question
Answer: Option D. -> YAC
Question 53. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
  1.    gel electrophoresis
  2.    photoreactivation
  3.    blotting
  4.    autoradiography
 Discuss Question
Answer: Option A. -> gel electrophoresis
Question 54. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
  1.    helicases
  2.    DNA gyrase
  3.    restriction endonucleases
  4.    DNA ligase
 Discuss Question
Answer: Option C. -> restriction endonucleases
Question 55. A genomic library is
  1.    a place where the information of the genetic organization of organisms are kept.
  2.    a book that describes how to isolate DNA from a particular organism
  3.    a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
  4.    a database where the sequence of an organism's genome is stored
 Discuss Question
Answer: Option C. -> a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
Question 56. For gene probes to be useful they must
  1.    be large enough to contain gene-specific sequences
  2.    None of these
  3.    both (a) and (b)
  4.    be labeled in some manner to allow detection
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 57. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
  1.    DNA ligase
  2.    Restriction endonuclease
  3.    DNA helicase
  4.    DNA polymerase
 Discuss Question
Answer: Option A. -> DNA ligase
Question 58. Bacterial cells protect their own DNA from degradation by restriction endonucleases by
  1.    methylating the DNA at the sites that the enzyme recognizes
  2.    not producing any restriction endonucleases
  3.    deleting all recognition sites from the genome
  4.    having anti restriction endonucleases
 Discuss Question
Answer: Option A. -> methylating the DNA at the sites that the enzyme recognizes
Question 59. Which type of restriction endonuclease cuts the DNA within the recognition site?
  1.    Type III
  2.    Type II
  3.    All of the these
  4.    Type I
 Discuss Question
Answer: Option B. -> Type II
Question 60. The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as
  1.    DNA probe
  2.    gene gun
  3.    inoculating needle
  4.    laser
 Discuss Question
Answer: Option B. -> gene gun

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