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MCQs

Total Questions : 62 | Page 2 of 7 pages
Question 11. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
  1.    DNA polymerase
  2.    Restriction endonuclease
  3.    DNA ligase
  4.    DNA helicase
 Discuss Question
Answer: Option C. -> DNA ligase
Answer: (c).DNA ligase
Question 12. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
  1.    southern blotting
  2.    northern blotting
  3.    polymerase chain reaction
  4.    DNA replication
 Discuss Question
Answer: Option C. -> polymerase chain reaction
Answer: (c).polymerase chain reaction
Question 13. The Southern blotting technique depends on
  1.    similarities between the sequences of probe DNA and experimental DNA
  2.    similarities between the sequences of probe RNA and experimental RNA
  3.    similarities between the sequences of probe protein and experimental protein
  4.    the molecular mass of proteins
 Discuss Question
Answer: Option A. -> similarities between the sequences of probe DNA and experimental DNA
Answer: (a).similarities between the sequences of probe DNA and experimental DNA
Question 14. Bacterial cells protect their own DNA from degradation by restriction endonucleases by
  1.    methylating the DNA at the sites that the enzyme recognizes
  2.    deleting all recognition sites from the genome
  3.    not producing any restriction endonucleases
  4.    having anti restriction endonucleases
 Discuss Question
Answer: Option A. -> methylating the DNA at the sites that the enzyme recognizes
Answer: (a).methylating the DNA at the sites that the enzyme recognizes
Question 15. Which type of restriction endonuclease cuts the DNA within the recognition site?
  1.    Type I
  2.    Type II
  3.    Type III
  4.    All of the these
 Discuss Question
Answer: Option B. -> Type II
Answer: (b).Type II
Question 16. In India first genetically engineered vaccine against HBV was developed by
  1.    Ranbaxy Pvt Ltd
  2.    Shantha Biotechnics Pvt Ltd
  3.    Dabur Pvt Ltd
  4.    Glaxo Pvt Ltd
 Discuss Question
Answer: Option B. -> Shantha Biotechnics Pvt Ltd
Answer: (b).Shantha Biotechnics Pvt Ltd
Question 17. The recombinant hGH lacks
  1.    terminal glutamine
  2.    terminal lysine
  3.    terminal methionine
  4.    terminal arginine
 Discuss Question
Answer: Option C. -> terminal methionine
Answer: (c).terminal methionine
Question 18. How is human insulin synthesized using recombinant DNA technology?
  1.    By using chemically synthesized DNA sequences for the two chains separately
  2.    By isolating DNA from the islets of Langerhans of pancreas
  3.    By using cDNA for insulin
  4.    By using chemically synthesized DNA sequences for the complete insulin protein
 Discuss Question
Answer: Option A. -> By using chemically synthesized DNA sequences for the two chains separately
Answer: (a).By using chemically synthesized DNA sequences for the two chains separately
Question 19. The two chains of insulin can be separated by detaching it from β-galactosidase using
  1.    hydrogen chloride
  2.    cyanogen bromide
  3.    peptidase
  4.    protease
 Discuss Question
Answer: Option B. -> cyanogen bromide
Answer: (b).cyanogen bromide
Question 20. Human growth hormone (hGH) is secreted by
  1.    pitutary gland
  2.    hypothalamus
  3.    pancreas
  4.    none of these
 Discuss Question
Answer: Option A. -> pitutary gland
Answer: (a).pitutary gland

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