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MCQs

Total Questions : 17 | Page 2 of 2 pages
Question 11.  Proteins separation can be carried out on the basis of
  1.    net charge
  2.    solubility in salt solutions
  3.    size or mass
  4.    all of these
 Discuss Question
Answer: Option D. -> all of these
Question 12.  Which of the following may be added to stabilize the protein after yeast cells disruption?
  1.    NaCl
  2.    Protease inhibitor
  3.    AMP
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 13.  Gel-filtration chromatography separates on the basis of
  1.    size and shape using porous beads packed in a column
  2.    size using porous beads packed in a column
  3.    shape using porous beads packed in a column
  4.    none of the above
 Discuss Question
Answer: Option A. -> size and shape using porous beads packed in a column
Question 14.  A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
  1.    1,000 units/mg
  2.    10,000 units/mg.
  3.    100,000 units/mg
  4.    1,000,000 units/mg
 Discuss Question
Answer: Option C. -> 100,000 units/mg
Question 15.  Affinity chromatography deals with the
  1.    specific binding of a protein constituents for another molecule
  2.    protein - protein interaction
  3.    protein - carbohydrate interaction
  4.    none of the above
 Discuss Question
Answer: Option A. -> specific binding of a protein constituents for another molecule
Question 16.  The best way to determine the location of protein in the purification scheme is to measure the
  1.    rate of ATP synthesis
  2.    changes in the refractive index
  3.    UV absorption
  4.    mass spectroscopy of the protein
 Discuss Question
Answer: Option A. -> rate of ATP synthesis
Question 17. . The best way to determine the location of protein in the purification scheme is to measure the
  1.    rate of ATP synthesis
  2.    changes in the refractive index
  3.    UV absorption
  4.    mass spectroscopy of the protein
 Discuss Question
Answer: Option A. -> rate of ATP synthesis

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