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MCQs

Total Questions : 56 | Page 3 of 6 pages
Question 21. Freezing technique is not preferred unless otherwise there is a need.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 22. _________________ is the main contaminant present in the resultant free extract from cells.
  1.    Chemicals
  2.    Simple sugars
  3.    Lipids
  4.    Nucleic acids
 Discuss Question
Answer: Option D. -> Nucleic acids
Answer: (d).Nucleic acids
Question 23. __________ is a method which is highly used for lab scale procedures than large scale work.
  1.    Ultrasonication
  2.    Homogenization
  3.    Freezing
  4.    Alkali treatment
 Discuss Question
Answer: Option A. -> Ultrasonication
Answer: (a).Ultrasonication
Question 24. Mortar and pestle, french press and homogenizer are widely used to lyse the cells by ultrasonication.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option B. -> False
Answer: (b).False
Question 25. Enzymes can be isolated from cell free extract by which one of the following methods?
  1.    Osmotic shock
  2.    Alkali treatment
  3.    Factors like pH, temperature and low ionic strength
  4.    Ultrasonication
 Discuss Question
Answer: Option C. -> Factors like pH, temperature and low ionic strength
Answer: (c).Factors like pH, temperature and low ionic strength
Question 26. Which of the following methods cannot be used to isolate enzymes from cell free extract?
  1.    Use of factors like pH and ionic strength
  2.    Use of chemicals
  3.    Homogenization
  4.    Use of temperature factor
 Discuss Question
Answer: Option C. -> Homogenization
Answer: (c).Homogenization
Question 27. Which one if the chemicals is not used to isolate enzymes from cell free extract?
  1.    1.4% buffered sucrose
  2.    Cetyl trimethyl ammonium bromide
  3.    Nucleases
  4.    Streptomycin sulphate
 Discuss Question
Answer: Option A. -> 1.4% buffered sucrose
Answer: (a).1.4% buffered sucrose
Question 28. The increase in pH affects the inter H₂ bonds and intramolecular hydrophobic bonds are majorly affected leading to denaturation of DNA.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option B. -> False
Answer: (b).False
Question 29. Increase or decrease in _________ leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.
  1.    temperature
  2.    ionic strength
  3.    pH
  4.    detergent use
 Discuss Question
Answer: Option C. -> pH
Answer: (c).pH
Question 30. ___________ is responsible for maintaining biologically native structure of an enzyme and even nucleic acid.
  1.    pH
  2.    Proton
  3.    ATP
  4.    Electrons
 Discuss Question
Answer: Option B. -> Proton
Answer: (b).Proton

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