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MCQs

Total Questions : 108 | Page 7 of 11 pages
Question 61. .  Which of the following is used to determine the appropriate stringency (Wallace rule)?
  1.    Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
  2.    Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
  3.    Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
  4.    Tm = 69.3° + 0.41 (%)G+C -650/1
 Discuss Question
Answer: Option C. -> Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Question 62. . Overwinding or overtightening of DNA during replication is caused and removed by
  1.    DNAb, DNA polymerase
  2.    DNAa, gyrase
  3.    DNAb, gyrase
  4.    Single stranded protein, DNAa
 Discuss Question
Answer: Option C. -> DNAb, gyrase
Question 63. . How does ethidium bromide interact with DNA?
  1.    It binds to adenine
  2.    It binds to phosphate
  3.    It intercalates between bases
  4.    It does not interact
 Discuss Question
Answer: Option C. -> It intercalates between bases
Question 64. . Number of times the two DNA strands are interwined in circular DNA and is known as
  1.    helix frequency
  2.    linking number
  3.    twisting number
  4.    none of these
 Discuss Question
Answer: Option B. -> linking number
Question 65. . The enzyme responsible for breaking the hydrogen bonds, and thus separating the DNA strands during DNA synthesis is
  1.    DNA polymerase
  2.    strandase
  3.    helicase
  4.    none of these
 Discuss Question
Answer: Option C. -> helicase
Question 66. . The size of a linear DNA fragment in a gel can be estimated by
  1.    fluorescence in UV light
  2.    the sedimentation coefficient
  3.    the time it takes the gel to run
  4.    comparison with a molecular weight standard such as Hind III digested phage lambda
 Discuss Question
Answer: Option D. -> comparison with a molecular weight standard such as Hind III digested phage lambda
Question 67. . Which of the following species lack a 'classical' histone H1?
  1.    Saccharomyces cerevisiae
  2.    Strongylocentrotus purpuratus
  3.    Xenopus laevis
  4.    Arabidopsis thaliana
 Discuss Question
Answer: Option A. -> Saccharomyces cerevisiae
Question 68. . Which of these drugs bind to DNA and cuts it, producing hydroxyl radicals?
  1.    Bleomycin
  2.    Erythromycin
  3.    Tunicamycin
  4.    All of these
 Discuss Question
Answer: Option A. -> Bleomycin
Question 69. . Which of the following are short nucleotides strands made by DNA polymerase working in the opposite direction of the replication fork?
  1.    Leading strands
  2.    Okazaki fragments
  3.    Primers
  4.    Lagging strand
 Discuss Question
Answer: Option B. -> Okazaki fragments
Question 70. . Which of the following is correct?
  1.    Replication starts at a single origin
  2.    Replication is bidirectional
  3.    Replication is semi conservative
  4.    All of the above
 Discuss Question
Answer: Option D. -> All of the above

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