Which of the following is incorrect about Yeast-two-hybrid screens?

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Which of the following is incorrect about Yeast-two-hybrid screens?

Options:

A . The yeast-two-hybrid system uses the transcription of a reporter gene driven by the Gal4 transcription factor to monitor whether or not two proteins are interacting

B . The DNA-binding domain chimeric protein will not bind upstream of the reporter gene

C . If the activation domain chimeric protein interacts with the DNA-binding domain chimeric protein, the reporter gene will be transcribed

D . Disadvantages of the method are that only pairwise interactions are tested, and not interactions that can only take place when multiple proteins come together, as well as a high false positive rate

Answer: Option B
Answer: (b).The DNA-binding domain chimeric protein will not bind upstream of the reporter gene

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