Questions and answers for exam Preparation

MCQs

Total Questions : 301 | Page 1 of 31 pages

Question 1. DNA- footprinting is a suitable technique for identifying which of the following?

Particular mRNA in mixture
Particular t-RNA in mixture
Introns within DNA
Protein binding site within DNA

Discuss Question

Answer: Option D. -> Protein binding site within DNA
Answer: (d).Protein binding site within DNA

Question 2. RAST test (radio allergosorbent test) is often used to detect

antibodies associated with allergies (IgE)
antigen associated with allergies (IgE)
bacteriophages
None of the above

Discuss Question

Answer: Option A. -> antibodies associated with allergies (IgE)
Answer: (a).antibodies associated with allergies (IgE)

Question 3. An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was more than one antigen
the antibody was impure
the temperature was too high
there was one antibody and one antigen

Discuss Question

Answer: Option A. -> there was more than one antigen
Answer: (a).there was more than one antigen

Question 4. What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

The ELISA would not develop when the substrate was added
The ELISA would develop normally
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of the above

Discuss Question

Answer: Option C. -> All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
Answer: (c).All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate

Question 5. What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Both (a) and (b)
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

Discuss Question

Answer: Option C. -> Both (a) and (b)
Answer: (c).Both (a) and (b)

Question 6. Individuals with PKU disease are mentally retarded unless

phenylalanine in the diet is restricted
tyrosine in the diet is restricted
homogentisic acid in the diet is restricted
none of the above

Discuss Question

Answer: Option A. -> phenylalanine in the diet is restricted
Answer: (a).phenylalanine in the diet is restricted

Question 7. Cyclins are proteins that

regulate ability of cells to invade tissue
regulate passage from one stage of cell division to another
regulate apoptosis of damaged cells
none of the above

Discuss Question

Answer: Option B. -> regulate passage from one stage of cell division to another
Answer: (b).regulate passage from one stage of cell division to another

Question 8. Protein folding is