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MCQs

Total Questions : 189 | Page 17 of 19 pages
Question 161. Transient gene silencing can be carried out by microinjecting ______
  1.    single stranded RNA
  2.    double stranded DNA
  3.    double stranded RNA
  4.    either double stranded DNA or RNA
 Discuss Question
Answer: Option C. -> double stranded RNA
Answer: (c).double stranded RNA
Question 162. Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.
  1.    lithium acetate
  2.    calcium phosphate
  3.    sodium chloride
  4.    aluminum sulphate
 Discuss Question
Answer: Option B. -> calcium phosphate
Answer: (b).calcium phosphate
Question 163. Where do P elements integrate into the genome?
  1.    At specifically defined sites
  2.    Randomly
  3.    Only at the ends of the genome
  4.    Integration depends on reaction conditions
 Discuss Question
Answer: Option B. -> Randomly
Answer: (b).Randomly
Question 164. Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?
  1.    Hypoxanthine and thymidine
  2.    Thymidine
  3.    Aminopterin and thymidine
  4.    Hypoxanthine, thymidine and aminopterin (HAT medium)
 Discuss Question
Answer: Option D. -> Hypoxanthine, thymidine and aminopterin (HAT medium)
Answer: (d).Hypoxanthine, thymidine and aminopterin (HAT medium)
Question 165. Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.
  1.    DNA polymerase, nucleus
  2.    DNA polymerase, cytoplasm
  3.    RNA polymerase, cytoplasm
  4.    RNA polymerase, nucleus
 Discuss Question
Answer: Option C. -> RNA polymerase, cytoplasm
Answer: (c).RNA polymerase, cytoplasm
Question 166. It is beneficial to use retrovirus for genetic manipulation of cells in the culture.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 167. Choose the incorrect statement in respect to MACs.
  1.    They are mammalian or human artificial chromosomes
  2.    Large stretches of DNA (in excess of 100 kb) can be inserted
  3.    A selectable marker is required
  4.    It can be maintained for long term
 Discuss Question
Answer: Option C. -> A selectable marker is required
Answer: (c).A selectable marker is required
Question 168. LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.
  1.    eukaryotic plasmid
  2.    prokaryotic plasmid
  3.    either eukaryotic or prokaryotic plasmid
  4.    yeast plasmid
 Discuss Question
Answer: Option B. -> prokaryotic plasmid
Answer: (b).prokaryotic plasmid
Question 169. The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?
  1.    Deletion of the packaging signal in the helper genome
  2.    Deletion of multiple parts of the helper genome
  3.    Division of the helper genome into several pieces
  4.    All of the mentioned
 Discuss Question
Answer: Option D. -> All of the mentioned
Answer: (d).All of the mentioned
Question 170. Technique of inserting Deoxyribonucleic Acid (DNA) into plants is known as
  1.    bio-injection
  2.    bio-fission
  3.    bio-genetic
  4.    bio-diffusion
 Discuss Question
Answer: Option B. -> bio-fission
Answer: (b).bio-fission

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