Which of the given statements is incorrect about Searching for orthologs to a pr

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Which of the given statements is incorrect about Searching for orthologs to a protein family in an EST database?

Options:

A . Searches of EST databases for matches to a query sequence routinely produce minimal amounts of output that must be searched manually for significant hits

B . ESTs with a high percent identity with the query sequence, a long alignment with the query sequence, and a very low E value of the alignment score represent groups of paralogous and orthologous genes

C . To identify orthologs as the most closely related sequence, ESTs were aligned using the amino acid alignment as a guide

D . To identify orthologs as the most closely related sequence, a phylogenetic tree was produced by the maximum likelihood method

Answer: Option A
Answer: (a).Searches of EST databases for matches to a query sequence routinely produce minimal amounts of output that must be searched manually for significant hits

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