Which of the following is used to determine the appropriate stringency (Wallace rule)? Options: A . &nbspTm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair) B . &nbspTm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair) C . &nbspTm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair) D . &nbspTm = 69.3° + 0.41 (%)G+C -650/1

Which of the following is used to determine the appropriate stringency (Wallace

Question

Which of the following is used to determine the appropriate stringency (Wallace rule)?

Options:

A . Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

B . Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)

C . Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

D . Tm = 69.3° + 0.41 (%)G+C -650/1

Answer: Option C
Answer: (c).Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

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