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Question
Which of the following is not included as a step in cloning in bacteriophage PI?
Options:
A .  The growth of vector is done as a plasmid by using pBR322 origin of replication
B .  Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated
C .  Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites
D .  Incubation is done with mutant lysogen which doesn’t contains head and tail proteins
Answer: Option C
Answer: (c).Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites

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