What is the next step after organic solvent fractionation with dry ice

Question

What is the next step after organic solvent fractionation with dry ice – ethanol – water mixture?

Options:

A . Refrigerated centrifugation at 3000 g for 15 mins

B . Drying in freezer

C . Supernatant fractionation with ethanol

D . Precipitated protein dissolved in 0.01 to 0.05 M cold buffer

Answer: Option A
Answer: (a).Refrigerated centrifugation at 3000 g for 15 mins

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