Linkers are often used in cloning. Choose the incorrect statement for linkers. Options: A . &nbspThese are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence B . &nbspThey are blunt ended molecules C . &nbspThey are ligated to staggered ended insert molecules by T4 DNA ligase D . &nbspAfter treatment with enzyme, both the ends of the linker are staggered

Linkers are often used in cloning. Choose the incorrect statement for linkers.

Question

Linkers are often used in cloning. Choose the incorrect statement for linkers.

Options:

A . These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence

B . They are blunt ended molecules

C . They are ligated to staggered ended insert molecules by T4 DNA ligase

D . After treatment with enzyme, both the ends of the linker are staggered

Answer: Option C
Answer: (c).They are ligated to staggered ended insert molecules by T4 DNA ligase

Was this answer helpful ?

Next Question

Question 1. A small circular DNA present in bacterial cells are called as....

Question 2. Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________....

Question 3. Insertion of DNA into lacZ gene may lead to disruption of the gene function.....

Question 4. Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and....is performed, this type of PCR is known as __________

Question 5. Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?....

Question 6. If genomic DNA has been inserted instead of the plasmid, what will happen?....

Question 7. It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using ___....and that have not. It is done by using __________

Question 8. If a restriction site is 6 nucleotides long, what are the chances of finding it in a vector?....

Question 9. Multiple cloning site (MCS) is defined as __________....

Question 10. Size of the vector is related to having a suitable single restriction site.....

Chapter 1 - GLOBAL STEEL SCENARIO & INDIAN STEEL INDUSTRY Part 1 : (13-04-2024) INDUSTRY AND COMPANY AWARENESS (ICA)

Direction Sense Test Part 1 Reasoning (Hindi)

Chapter 1 - RMHP / OHP / OB & BP Part 1 : (14-02-2024) GPOE

Cube & Cuboid Part 1 Reasoning (Hindi)

Data Interpretation (DI) Basic Concept Reasoning (Hindi)

Counting Figures Part 1 Counting Of Straight Lines Reasoning (Hindi)

Sail E0 free webinar Webinars

Real Numbers Part 7 Class 10 Maths

Real Numbers Part 1 Class 10 Maths

Polynomials Part 1 Class 10 Maths

Pie Chart - Test Paper 1 Data Interpretation Reasoning & DI

Tabulation - Test Paper 2 Data Interpretation Reasoning & DI

Test Paper 1 Direction Sense Test Reasoning & DI

ICA , RDIC,GFM Free Modal Test Paper - Non - Technical Branch Old Syllabus 2022 - SAIL Junior Officer E0

GFM Combined 1 Free Revision Test SAIL JO E-0