Questions and answers for exam Preparation

MCQs

Total Questions : 32 | Page 3 of 4 pages

Question 21. Which of the following is not a step in producing penicillin -G-amidase?

Cleavage by Hind III
Replacement of asparagine residue with serine present in the native enzyme
Cleaved fragments cloned into a cosmid vector
Penicillin-G-amidase gene transferred on to pBR322 plasmids

Discuss Question

Answer: Option B. -> Replacement of asparagine residue with serine present in the native enzyme
Answer: (b).Replacement of asparagine residue with serine present in the native enzyme

Question 22. Synthetic polymers or oligomers with enzyme-like activities possess two structural entities, a substrate-binding site and a catalytically effective site are referred to as ___________

co-factors
co-enzymes
biosensors
synzymes

Discuss Question

Answer: Option D. -> synzymes
Answer: (d).synzymes

Question 23. Protein engineering directed at subtilisin has been aimed to improve the activity in detergents by stabilizing it at even higher temperatures, pH and oxidant strength with some concerned alterations.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 24. Which of the following is not a step involved in site directed mutagenesis using a hypothetical example?

Derive the primary structure of the enzyme from the DNA sequence
Identification of mutant DNA by hybridization with radioactively labelled oligonucleotides
Cleavage by Hind III
Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme

Discuss Question

Answer: Option C. -> Cleavage by Hind III
Answer: (c).Cleavage by Hind III

Question 25. Which of the following techniques may not be used to produce thermophilic enzymes?

Internal salt link
Extra internal hydrophobic residues
Suitable exterior substitutions
Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme

Discuss Question

Answer: Option D. -> Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme
Answer: (d).Primary structure of putative enzyme is proposed with an asparagine residue replacing the serine present in the native enzyme

Question 26. Which of the following is not true for synzymes?

They are referred to as artificial enzymes
They possess two structural entities which are a substrate binding site and a catalytically effective site
They do not obey the Miachelis-Menten kinetics
Producing catalytic site is difficult

Discuss Question

Answer: Option C. -> They do not obey the Miachelis-Menten kinetics
Answer: (c).They do not obey the Miachelis-Menten kinetics

Question 27. Derivative of myoglobin acts as an effective natural ascorbate oxidase.